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目的:探讨前列腺素DP受体(DPR)对小鼠睡眠-觉醒调节的影响。方法:在苯巴比妥麻醉下,于DPR基因敲除(KO)小鼠及其野生型( WT)小鼠大脑皮层及颈部肌肉分别植入脑电(Electroencepha-logram,EEG)和肌电(Electromyogram, EMG)电极,用EEG/EMG睡眠记录系统于20 00时开始连续记录24小时两种小鼠的脑电和肌电波,并用SLEEPSIGN软件进行分析。结果:两种小鼠表现出相同的睡眠-觉醒节律,且明时(8 00 -20 00)及暗时(20 00 -8 00)时相内两种小鼠的非快动眼(NREM)睡眠和快动眼(REM)睡眠总量无差异。但与WT小鼠相比,DPR-KO小鼠明时内的觉醒频率显著增高,NREM睡眠的平均时程显著缩短;且DPR-KO小鼠睡眠呈现低活性的θ波和高活性的δ波。结论:DPR在介导前列腺素D2诱导的睡眠中起着关键性调节作用,缺乏DPR将导致小鼠呈现低强度片段化的NREM睡眠和高警戒状态的REM睡眠。
Objective: To investigate the effect of prostaglandin DP receptor (DPR) on sleep-wake regulation in mice. METHODS: Under phenobarbital anesthesia, electroencephalogram (EEG) and myoelectricity (EEG) were implanted into the cerebral cortex and neck muscle of DPR knockout (KO) mice and their wild type (WT) Electroencephalogram (EMG) electrodes were used to record the EEG and EMG of both mice for 24 hours continuously using the EEG / EMG sleep recording system at 2000 hours and analyzed by SLEEPSIGN software. RESULTS: Both mice showed the same sleep-wake rhythm and were non-fast moving eyes (NREM) in both mice in the bright (00:00 to 00:00) and dark (20000 to 80000) phases There was no difference in total sleep between REM sleep and REM sleep. However, compared with WT mice, DPR-KO mice had a significantly higher frequency of wakefulness in the middle of the day and a significantly shorter average duration of NREM sleep. In DPR-KO mice, low activity θ wave and high activity δ wave . CONCLUSIONS: DPR plays a key regulatory role in mediating prostaglandin D2-induced sleep, and lack of DPR results in mice presenting with low-intensity, fragmented NREM sleep and high alert REM sleep.