免疫磁珠技术已应用于免疫检测、细胞分离、蛋白质纯化等方面,并显示了良好的前景.本实验采用免疫磁珠技术检测外周血中卵巢癌细胞,1.提取外周血单核细胞,2. 建立血行转移模型:将卵巢上皮性癌细胞株3AO细胞极限稀释,按比例加入获得的单核细胞中,单核细胞与癌细胞比例分别为10~4:1,5×10~4 :1,10~5:1,5×10~5:1,获得卵巢癌血行转移模型,3. 制备免疫磁珠:将磁珠清洗干净后,用不同PH值缓冲液恢复原体积,取我中心制备的卵巢癌单抗COC183-B2,用缓冲液稀释,加偶联剂碳二亚胺,混匀后加入清洗好的磁珠,再次混匀,置37℃摇床缓慢振荡2小时,用洗液清洗后,获得包被单抗的免疫磁珠.4. 免疫检测:在建立的卵巢癌血行转移模型中加入包被单抗的免疫磁珠,磁珠与细胞比例为10:1,混匀,37℃摇床缓慢 Immunomagnetic beads technology has been applied in immunological detection, cell separation, protein purification, etc., and shows a good prospect.In this study, immunomagnetic beads technology was used to detect ovarian cancer cells in peripheral blood, 1. Extract peripheral blood mononuclear cells, 2 To establish a hematogenous metastasis model, the ovarian epithelial cancer cell line 3AO was diluted to the limit and added to the obtained mononuclear cells in a ratio of 10: 4: 1, 5 × 10-4: 1 , 10 ~ 5: 1, 5 × 10 ~ 5: 1, to obtain ovarian cancer hematogenous metastasis model, 3. Preparation of immunomagnetic beads: After the magnetic beads were washed with different pH buffer to restore the original volume, take my center preparation Of ovarian cancer monoclonal antibody COC183-B2, diluted with buffer solution, add coupling agent carbodiimide, mix well and then add the cleaned magnetic beads, mix again and set at 37 ℃ shaker for 2 hours with slow shaking, After washing, the immunomagnetic beads coated with monoclonal antibody were obtained.4.Immunity detection: Immunomagnetic beads coated with monoclonal antibody were added into the established model of hematogenous metastasis of ovarian cancer, the ratio of magnetic beads to cells was 10: 1, Shaker slowly