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目的了解医院分离的多重耐药的鲍曼不动杆菌(ABA)氨基糖苷类药物各种耐药相关基因存在状况,探讨ABA多药耐药机制。方法收集20株分离自2010年1-12月岳阳市二人民医院患者临床分离的ABA,采用纸片扩散法测定16种抗菌药物的敏感性,采用PCR法检测armA、rmtA、rmtBr、mtC、rmtD、npmAa、ac(3)-Ⅰa、ac(3)-Ⅱ、aac(6′)-Ⅱ、aac(6′)-Ⅰba、ac(6′)-Ⅰa、ant(3“)-Ⅰ、ant(2”)-Ⅰ等16SrRNA甲基化酶与氨基糖苷类修饰酶基因。结果 1株检出16SrRNA甲基化酶基因(armA),15株检出氨基糖苷类修饰酶基因[其中aac(3)-Ⅰ为80%、ant(2“)-Ⅰ为80%、aac(3)-Ⅱ为40%、aac(6′)-Ⅰb为15%]。结论鲍曼不动杆菌的多重耐药与氨基糖苷类修饰酶基因和16-SrRNA甲基化酶基因相关。鲍曼不动杆菌存在新的氨基糖苷类药物耐药机制。
Objective To understand the existence of multidrug resistance-related genes in multiple drug-resistant Acinetobacter baumannii (ABA) aminoglycosides isolated in hospital and explore the mechanism of ABA multidrug resistance. Methods Twenty clinical isolates of ABA were collected from two People’s Hospital of Yueyang City from January to December in 2010. The sensitivity of 16 antimicrobial agents was determined by disk diffusion method. The levels of armA, rmtA, rmtBr, mtC, rmtD (3) - Ⅰa, ac (3) - Ⅱ, aac (6 ’) - Ⅱ, aac (6’) - Ⅰba, ant (2 ”) - Ⅰ 16SrRNA methylase and aminoglycoside modified enzyme gene. Results A gene of 16SrRNA methylase (armA) and aminoglycoside modified enzyme (15%) were detected in 1 strain (80% of aac (3) -I and 80% of ant (3) 40% for a-Ⅱ and 15% for aac (6 ’) -Ib.Conclusion The multi-drug resistance of Acinetobacter baumannii is related to the aminoglycoside-modifying enzyme gene and 16-SrRNA methylase gene There is a new mechanism of aminoglycoside resistance in M.