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目的 :研究人肝癌smmc 772 1裸鼠模型 ,注射“99 star”及无水酒精前后 ,端粒酶hTRT(hu mantelomerasereversetranscriptase)表达率的变化及其与疗效的关系。 方法 :人肝smmc 772 1裸鼠 19只 ,在B超引导下经皮穿剌 ,分别予注射“99 star”(11只 )、无水酒精 (5只 )、生理盐水 (3只 ) ,注射前后抽取肝癌细胞 ,采用原位杂交法检测端粒酶hTRT表达率的变化。结果 :“99 star”及无水酒精组与生理盐水组疗效相比 ,P <0 .0 5 ,差异有显著性 ,而“99 star”与无水酒精相比疗效相当 ,差异无显著性。 11只注射“99 star”、5只注射无水酒精、3只注射生理盐水的裸鼠 ,hTRT由注射前阳性转变为阴性的例数分别为 :7/ 9、4/ 4、0 / 2 ,前两者与后者转为阴性例数相比较差异有显著性。“99 star”与无水酒精治疗裸鼠肝癌的疗效相当 ,两者注射后hTRT转为阴性者均较未转阴性者疗效好。结论 :“99 star”是一可供B超介导治疗裸鼠肝癌的有效药物 ,检测端粒酶hTRT对判断“99 star”治疗裸鼠疗效有重要意义
OBJECTIVE: To investigate the changes of telomerase hTRT (hu-mantelomerasereversetranscriptase) expression in human hepatocellular carcinoma smmc 772 1 nude mice model before and after injection of “99 star” and anhydrous alcohol and its relationship with therapeutic effect. Methods: Nineteen human liver smmc 772 1 nude mice were percutaneously punctured with B ultrasound and injected with “99 star” (11), anhydrous alcohol (5), saline (3) Liver cancer cells were harvested before and after, and the expression of telomerase hTRT was detected by in situ hybridization. Results: There was significant difference between the “99 star” group and the anhydrous alcohol group and saline group (P <0.05), while the “99 star” group had the same effect as the anhydrous alcohol group with no significant difference. There were 11 cases of “99 star” injection, 5 cases of injection of anhydrous alcohol and 3 cases of normal saline injected nude mice respectively. The number of hTRT from positive to negative before injection was 7/9, 4/4, 0/2, The difference between the former two and the latter was negative. “99 star” and ethanol treatment of liver cancer in nude mice the same effect, both after injection of hTRT turned negative were better than non-negative. Conclusion: “99 star” is an effective drug for B-mediated transplanted hepatocarcinoma in nude mice. Detection of telomerase hTRT is of great significance in judging the therapeutic effect of “99 star” on nude mice