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【目的】了解八门湾红树林海漆林区土壤中可培养芽胞杆菌资源的多样性。【方法】采用水浴处理与直接涂布相结合的方法选择性分离土壤中的芽胞杆菌;利用16S rDNA PCR-RFLP与16S rDNA序列分析技术研究可培养芽胞杆菌资源的遗传多样性和系统发育关系。【结果】16S rDNA PCR-RFLP酶切图谱UPGMA聚类分析表明,在100%的相似性水平上,分离的155株芽胞杆菌分属21个遗传类群,显示了较为丰富的遗传多样性;由21种遗传类型代表菌株的16S rDNA序列分析结果得知,这些芽胞杆菌主要分布在Bacillaceae和Paenibacillaceae科下的Bacillus、Halobacillus、Virgibacillus和Paenibacillus 4个属,其中Bacillus为优势属;有8株芽胞杆菌的16S rDNA序列与数据库中相应模式菌株的最大相似性在95.1%-99.0%之间。【结论】八门湾红树林土壤可培养芽胞杆菌有着较为丰富的遗传多样性,并存在新的芽胞杆菌物种资源。
【Objective】 The objective of this study was to understand the diversity of Bacillus subtilis resources in soils of mangrove forest in Bayimen Bay. 【Method】 Bacillus subtilis was isolated by the combination of water bath and direct coating. The 16S rDNA PCR-RFLP and 16S rDNA sequence analysis were used to study the genetic diversity and phylogeny of Bacillus caniculture. 【Result】 The UPGMA cluster analysis of 16S rDNA PCR-RFLP digestion showed that 155 Bacillus isolated belong to 21 genetic groups at the level of 100% similarity and showed rich genetic diversity. The results of 16S rDNA sequence analysis showed that these Bacillus were mainly distributed in 4 genera of Bacillus, Halobacillus, Virgibacillus and Paenibacillus in Bacillaceae and Paenibacillaceae, of which Bacillus was the dominant genus, and 8 Bacillus strains of 16S The maximum similarity between the rDNA sequence and the corresponding model strain in the database was between 95.1% and 99.0%. 【Conclusion】 Bacillus can be cultured in mangroves of Bayimen Bay is rich in genetic diversity, and there is a new Bacillus species resources.