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近10年对应用IUD和服复方避孕药妇女免疫球蛋白水平的研究引起了一些注意,多数学者试图确定是IUD的作用方式或激素对免疫球蛋白的影响。本文是首始研究IUD使用者宫颈粘液免疫球蛋白水平。作者通过琼脂凝胶单纯放射免疫扩散法测定50例应用带尾Cu-IUD,20例服复方避孕药和20例没有避孕措施的生育年龄妇女的血清和宫颈粘液免疫球蛋白水平。特异性抗IgA、IgG和IgM抗血清是从Dakopatts购得,琼脂中抗原的最适合浓度的建立,是经过最初实验改变凝胶中抗血清的浓度,直至取得各种抗体最适合的环直经,即IgA1.5μl/cm~2,IgG0.65μl/cm~2、IgM0.5μl/cm~2。培养基大小为8×8cm,孔直径为2.5cm,培养基于室温
In the past 10 years, studies on the immunoglobulin levels of women using compound oral contraceptives and IUD have drawn some attention. Most scholars are trying to determine whether the mode of action of IUDs or the effects of hormones on immunoglobulins. This article is the first to study cervical mucus immunoglobulin levels in IUD users. The authors used agar gel radioimmunodiffusion method to determine the serum and cervical mucus immunoglobulin levels in 50 cases of Cu-IUD with tail, 20 cases of contraceptives and 20 women of childbearing age without contraception. Specific anti-IgA, IgG and IgM antisera were purchased from Dakopatts and the optimal concentration of antigen in the agar was established by initially experimentally varying the concentration of antisera in the gel until the optimal ring diameter for each antibody was obtained , Namely IgA1.5μl / cm2, IgG0.65μl / cm2, IgM0.5μl / cm2. The medium size was 8 × 8 cm, the hole diameter was 2.5 cm, and the culture was at room temperature