Haynaldia villosa (2n = 2X = 14, VV), a relative of wheat, plays important roles in wheat improvement mainly owing to itsdisease resistance. Powdery mildew resistance gene Pm21 has been successfully transferred into wheat by CytogeneticInstitute, Nanjing Agricultural University, China, and is widely used in the current wheat breeding programs. In thisresearch, our objective is to further transfer and utilize the beneficial genes such as eye-spot resistance, yellow rustresistance, and gene of the tufted bristles on the glume ridge (a remarkable morphology) mapped on 2V of Haynaldiavillosa. A disomic addition line with gametocidal chromosome 3C of Aegilops triuncialis added in Norin-26 was crossedto the wheat-H. villosa disomic substitution 2V(2D) and the hybrid F1 was then self-crossed. Chromosome C-banding,genomic in situ hybridization (GISH), and meiotic analysis in combination with molecular markers were applied to detectthe chromosome variations derived from hybrids F2 and F3. To date, four translocations including one small segmentaltranslocation T6BS·6BL-2VS, two whole arm translocations (preliminarily designed as T3DS·2VL and T2VS·7DL) and oneintercalary translocation T2VS·2VL-W-2VL, one deletion Del. 2VS·2VL-, one monotelosomic Mt2VS, and one iso-chromosome 2VS·2VS line have been developed and characterized. One wheat SSR marker Xwmc25-120 tagging 2VS andone wheat STS marker NAU/STSBCD135-1 (2BL) tagging 2VL were successfully used to confirm the alien chromosomesegments involved in the seven lines. The tufted bristles on the glume ridge appeared in lines T2VS·7DL, Mt2VS,2VS·2VS as well as the parent DS2V(2D), whereas in T3DS·2VL, this trait did not appear. The gene controlling the tuftedbristles was located on 2VS. Gametocidal chromosome 3C of Aegilops triuncialis could successfully induce chromosome2V structural changes. Hayley dill villosa (2n = 2X = 14, VV), a relative of wheat, plays important roles in wheat improvement mainly due to itsdisease resistance. Powdery mildew resistance gene Pm21 has been successfully transferred into wheat by Cytogenetic Institute, Nanjing Agricultural University, China, and is this used in the current wheat breeding programs. our this is to further transfer and utilize the beneficial genes such as eye-spot resistance, yellow rustresistance, and gene of the tufted bristles on the glume ridge (a remarkable morphology) mapped on 2V of Haynaldia villosa. A disomic addition line with gametocidal chromosome 3C of Aegilops triuncialis added in Norin-26 was crossed to the wheat-H. villosa disomic substitution 2V (2D) and the hybrid F1 was then self-crossed. Chromosome C-banding, genomic in situ hybridization (GISH), and meiotic analysis in combination with molecular markers were applied to detect the chromosomes variants derived from hybrids F2 and F3. To da te, four translocations including one small segmental transposition T6BS · 6BL-2VS, two whole arm translocations (preliminarily designed as T3DS · 2VL and T2VS · 7DL) and oneintercalary translocation T2VS · 2VL-W-2VL, one deletion Del. 2VS · 2VL-, one monotelosomic Mt2VS, and one iso-chromosome 2VS · 2VS line have been developed and characterized. One wheat SSR marker Xwmc25-120 tagging 2VS and one wheat STS marker NAU / STSBCD135-1 (2BL) tagging 2VL were successfully used to confirm the alien chromosomes profiles involved in the seven lines. The tufted bristles on the glume ridge appeared in lines T2VS · 7DL, Mt2VS, 2VS · 2VS as well as the parent DS2V (2D), while in T3DS · 2VL, this trait did not appear. The gene controlling the tuftedbristles was located on 2VS. Gametocidal chromosome 3C of Aegilops triuncialis could successfully induce chromosome2V structural changes.