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目的建立透骨草药材来源中狭山野豌豆Vicia amoena超高效液相色谱(UPLC)指纹图谱,并与其余5种来源品种进行比较,为透骨草药材质量控制提供参考依据。方法用Eclipse Plus C_(18) RRHD(50 mm×2.1 mm,1.8μm)色谱柱,以乙腈-0.1%甲酸溶液为流动相梯度洗脱,体积流量0.2 m L/min;柱温30℃;检测波长340 nm。测定10个不同产地狭山野豌豆指纹图谱,采用中药色谱指纹图谱相似度评价系统(2004A版)计算相似度,并用对照品和液质联用技术指认色谱峰;同条件下比较鉴别透骨草药材其他5种来源品种。结果建立了狭山野豌豆指纹图谱,采用超高效液相色谱-电喷雾-四级杆-飞行时间串联质谱仪(UPLC-ESI-Q-TOF-MS)对14个共有峰中的6个峰进行初步归属,确定为绿原酸、杨梅苷、金丝桃苷、异槲皮苷、山柰苷、槲皮苷;透骨草药材其他5种来源品种与狭山野豌豆指纹图谱的共有模式明显不同。结论该方法重现性好、特征性强,可用于透骨草药材全面质量评价。
OBJECTIVE To establish a HPLC fingerprint of Vicia amoena in Pekingia flavescens and compare it with the other five cultivars to provide a reference for the quality control of the tragacanth. Methods The mobile phase was eluted with a mobile phase of acetonitrile-0.1% formic acid at a flow rate of 0.2 mL / min using an Eclipse Plus C_ (18) RRHD column (50 mm × 2.1 mm, 1.8 μm) Wavelength 340 nm. The fingerprints of 10 wild pea cultivars in different habitats were determined and the similarity was calculated by using the similarity evaluation system of Chinese traditional medicine chromatographic fingerprinting (2004A version). The chromatographic peaks were identified by using the reference substance and LC-MS technique. Other five kinds of source varieties. Results The fingerprints of Pinus sibirica were established and the six peaks of 14 common peaks were determined by UPLC-ESI-Q-TOF-MS using ultra performance liquid chromatography-electrospray-quadrupole-time of flight tandem mass spectrometry Initial ownership, identified as chlorogenic acid, myricitrin, hyperoside, isoquercitrin, behenyl glycosides, quercitrin; the other five sources of species of traditional Chinese medicine and the wild mountain pea fingerprinted common pattern significantly different . Conclusion The method has good reproducibility and strong characteristic, and can be used to evaluate the total quality of