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腺苷作为一种神经调质，能抑制多种递质的释放，发挥负反馈调节作用。用外淋巴灌流给药方法，观察了腺苷及其摄取抑制剂双嘧啶氨醇、受体拮抗剂茶碱对豚鼠复合听神经动作电位（ＣＡＰ）、微音器电位（ＣＭ）、蜗内直流电位（ＥＰ）的影响。结果表明０．１ｍｍｏｌ／Ｌ腺苷及双嘧啶氨酸使ＣＡＰ、ＣＭ振幅降低，输入／输出（Ｉ／Ｏ）曲线右移，ＣＡＰ潜伏期延长。灌流１ｍｍｏｌ／Ｌ茶碱则出现相反改变。上述变化在人工外淋巴冲洗灌流后可基本恢复正常。ＥＰ及缺氧法观察所得负ＥＰ（Ｎ－ＥＰ）均无明显改变，但腺苷灌流后，ＥＰ对强声刺激给－撤声（ｏｎ－ｏｆｆ）时的反应减弱。结果提示，腺苷可能作为抑制性调质在耳蜗起着负反馈调节作用。
Adenosine as a neuromodulator, can inhibit the release of a variety of neurotransmitters, play a negative feedback regulation. To observe the effects of adenosine and its uptake inhibitor, dipyrimidinosine and theophylline, a receptor antagonist on guinea pig composite auditory nerve action potential (CAP), microphter potential (CM), intracochlear DC potential (EP) of the impact. The results showed that 0.1 mmol / L adenosine and dipyrimidine reduced the amplitude of CAP and CM, shifted the input / output (I / O) curve to the right, and prolonged the incubation period of CAP. Perfusion 1mmol / L theophylline appears the opposite change. The above changes in the artificial perilymph perfusion can basically return to normal. EP and hypoxia observed negative EP (EP) no significant change, but after adenosine perfusion, EP on strong acoustic stimulation - on-off (response) when weakened. The results suggest that adenosine may play a negative feedback regulatory role in the cochlea as an inhibitory mediator.