背景:CD62p作为血小板活化的分子标记物,不仅反应了血小板活化程度及功能状态,还介导血小板与中性粒细胞及内皮细胞的粘附功能,加重脑缺血后再灌注损伤。目的:观察中药脑宁康颗粒对经脑缺血预适应和脑缺血再灌注处理后的大鼠血小板膜表面受体水平的影响,并与西药阿司匹林进行比较。设计:随机对照实验。单位:上海中医药大学附属曙光医院脑病中心。材料:实验于1998-07／1999-02在山东医科大学完成。选择雄性 Wistar大鼠48只,随机分为模型组,假手术组,阿司匹林组和脑宁康组,每组12只。每组取6只用脑缺血预适应方法造模。其余6只用脑缺血再灌注方法造模。脑宁康颗粒由川芎,蚤休,海藻等成分组成,生药含量为5 g／g。方法:根据Pulsinelli四管法及Liu法分别制作大鼠脑缺血预适应和脑缺血再灌注模型,分别于缺血再灌注24 h后在麻醉状态下断头取血, 以荧光抗体标记法在流式细胞仪上测定并比较脑缺血预适应和脑缺血再灌注大鼠血小板膜表面受体水平。主要观察指标:脑缺血预适应和脑缺血再灌注大鼠血小板膜表面受体水平。结果:48只大鼠均进入结果分析。①缺血再灌注后24 h,两种方法模型组大鼠的CD 62P值均明显升高(P<0．01),而脑宁康和阿司匹林治疗组CD 62P虽有增加,但不明显(P>0．05)。②脑缺血预适应模型中除假手术组外,各组CD 62P值均较脑缺血再灌注模型变化幅度小。③在两种模型中,脑宁康颗粒和阿司匹林均对CD 62P有良性效应, 其中脑宁康组略优于阿司匹林组,但差异无显著性意义(P>0．05)。结论:脑宁康颗粒对脑缺血再灌注和脑缺血预适应状态下的CD 62P表达均有良性效应,其强度略高于阿司匹林,说明脑宁康颗粒通过干扰血小板活化,抑制其聚集、改善脑局部微循环,从而保护脑神经元。 BACKGROUND: CD62p, as a molecular marker of platelet activation, not only reflects the activation and functional status of platelets, but also mediates the adhesion of platelets to neutrophils and endothelial cells and aggravates cerebral ischemia-reperfusion injury. OBJECTIVE: To observe the effect of Naoningkang Granules on platelet membrane surface receptors in rats after cerebral ischemic preconditioning and cerebral ischemic reperfusion treatment, and compare them with western medicine aspirin. Design: Randomized controlled trials. Unit: Center of Encephalopathy, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine. MATERIALS: The experiment was performed at Shandong Medical University from July 1998 to February 1999. 48 male Wistar rats were randomly divided into model group, sham operation group, aspirin group and Naoningkang group, 12 in each group. Six rats in each group were modeled by cerebral ischemic preconditioning. The remaining 6 mice were modeled by cerebral ischemia-reperfusion. Naoningkang granules are composed of Chuanxiong, Qixiu, and seaweed. The crude drug content is 5 g/g. METHODS: Rat cerebral ischemic preconditioning and cerebral ischemic reperfusion models were established according to the Pulsinelli four-tube method and Liu method respectively. The rats were decapitated under anesthesia 24 h after ischemia and reperfusion, and they were labeled with fluorescent antibody. The levels of platelet membrane receptors in rats with cerebral ischemic preconditioning and cerebral ischemia-reperfusion were measured and compared on flow cytometry. MAIN OUTCOME MEASURES: Levels of platelet membrane surface receptors in rats with cerebral ischemic preconditioning and cerebral ischemia-reperfusion. Results: All 48 rats were involved in the result analysis. 1 At 24 h after ischemia and reperfusion, the CD62P values ​​of the two models of the model group were significantly increased (P<0.01), while the CD62P in the Naoningkang and aspirin treatment groups increased, but not significantly ( P>0.05). 2 Except for the sham operation group, the CD62P values ​​in each group were smaller than those in the cerebral ischemia-reperfusion model. 3 In both models, both Naoningkang Granules and aspirin had a benign effect on CD62P, and the Naoningkang group was slightly better than the aspirin group, but the difference was not significant (P>0.05). Conclusion: Naoningkang Granule has a benign effect on the expression of CD62P under cerebral ischemic reperfusion and cerebral ischemic preconditioning. Its intensity is slightly higher than that of aspirin, indicating that Naoningkang Granule inhibits its aggregation through interfering with platelet activation. Improve brain microcirculation, thereby protecting brain neurons.