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目的探讨人巨细胞病毒(HCMV)感染儿童的白细胞介素12(IL-12)亚基p40 3’非翻译区+1188(A/C)位点基因多态性与HCMV感染的关系。方法应用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)和DNA测序的方法,检测125例HCMV活动性感染儿童、108例HCMV潜伏性感染儿童和110例健康儿童IL-12p40 3’非翻译区+1188(A/C)位点的基因型,采用荧光定量聚合酶链反应(FQ-PCR)测定HCMV活动性感染儿童尿液HCMV DNA载量。结果 HCMV活动性感染组、潜伏性感染组和对照组之间AA、AC、CC基因型分布差异有统计学意义(χ2=10.542,P=0.032);HCMV活动性感染组A、C等位基因分布频率分别为52.8%、47.2%,潜伏性感染组分别为63.4%、36.6%,对照组分别为67.7%、32.3%,3组的差异有统计学意义(χ2=11.777,P=0.003);HCMV活动性感染组与非活动性感染组(潜伏性感染组+对照组)的等位基因分布频率差异有统计学意义(χ2=10.930,P=0.001;ORA=0.805,95%CI:0.703~0.922;ORC=1.372,95%CI:1.141~1.650);不同基因型HCMV活动性感染儿童的尿HCMVDNA载量差异有统计学意义(F=9.325,P=0.006),其中CC基因型患儿尿HCMV DNA载量高于AA基因型(P=0.008)。结论 HCMV感染与IL-12亚基p40 3’非翻译区+1188(A/C)位点基因多态性具有相关性,其中C等位基因可能是HCMV活动性感染的遗传易感基因,携带C等位基因的个体可能更利于病毒的复制。
Objective To investigate the relationship between polymorphism of p40 3 ’untranslated region + 1188 (A / C) gene of interleukin 12 (IL-12) subunit and HCMV infection in children with human cytomegalovirus (HCMV) infection. Methods 125 cases of HCMV active infection, 108 cases of HCMV latent infection and 110 healthy children were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing. IL-12p40 3 ’untranslated region + 1188 (A / C) loci genotypes were detected by fluorescence quantitative polymerase chain reaction (FQ-PCR) HCMV activity of children infected with urine HCMV DNA load. Results The distribution of AA, AC and CC genotypes between HCMV active infection group, latent infection group and control group was statistically significant (χ2 = 10.542, P = 0.032). The A and C alleles of HCMV active infection group The frequency of distribution was 52.8% and 47.2% respectively. The latent infection was 63.4% and 36.6% respectively, while the control group was 67.7% and 32.3% respectively. There was significant difference between the three groups (χ2 = 11.777, P = 0.003). There was significant difference in allele frequency between HCMV active infection group and inactive infection group (latent infection group + control group) (χ2 = 10.930, P = 0.001; ORA = 0.805, 95% CI: 0.703 ~ 0.922, ORC = 1.372, 95% CI: 1.141-1.650). The urinary HCMVDNA load in children with active HCMV infection was significantly different (F = 9.325, P = 0.006) HCMV DNA load was higher than AA genotype (P = 0.008). Conclusion The HCMV infection is associated with the gene polymorphism of p40 3 ’untranslated region + 1188 (A / C) in IL-12 subunit. The C allele may be a genetic predisposition to active HCMV infection, Individuals at the C allele may be more favorable to virus replication.