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目的：观察不同浓度的血管紧张素Ⅱ（ＡｎｇⅡ）刺激后系膜细胞ＡｎｇⅡ受体基因表达和分泌纤维连接蛋白（ＦＮ）的情况。方法：从６月龄人胎肾培养系膜细胞，经ＡｎｇⅡ刺激后用逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）方法检测胎肾系膜细胞ＡＴ１和ＡＴ２受体ｍＲＮＡ的表达情况，分别用ＥＬＩＳＡ和ＲＴ－ＰＣＲ方法检测系膜细胞产生的ＦＮ和基因表达。结果：①经１０－８、１０－７、１０－６和１０－５ｍｏｌ／Ｌ的ＡｎｇⅡ刺激４８ｈ后，系膜细胞的ＡＴ１受体ｍＲＮＡ表达均有升高，并呈一定的浓度依赖性；而ＡＴ２受体ｍＲＮＡ仅在１０－５ｍｏｌ／Ｌ浓度的ＡｎｇⅡ刺激下才有微弱表达。②１０－７、１０－６和１０－５ｍｏｌ／Ｌ的ＡｎｇⅡ能明显促进系膜细胞产生ＦＮ和ＦＮ的ｍＲＮＡ表达的上调，并与ＡｎｇⅡ刺激浓度呈正相关。结论：ＡｎｇⅡ刺激后可使系膜细胞的ＡＴ１受体基因表达和ＦＮ的产生明显增加，并呈一定的浓度依赖性，而ＡｎｇⅡ高浓度可以使ＡＴ２受体表达上调
Objective: To observe the expression of angiotensin Ⅱ receptor gene and the secretion of fibronectin (FN) in mesangial cells stimulated with different concentrations of angiotensin Ⅱ (Ang Ⅱ). Methods: The mesangial cells were cultured from 6-month-old human fetal kidney. The expressions of AT1 and AT2 receptor mRNA in fetal renal mesangial cells were detected by reverse transcription-polymerase chain reaction (RT-PCR) ELISA and RT-PCR methods to detect mesangial cells FN and gene expression. RESULTS: ① After stimulated with AngII for 10-8,10-7,10-6 and 10-5mol / L for 48h, the mRNA expression of AT1 receptor in mesangial cells increased in a concentration-dependent manner. AT2 receptor mRNA was only weakly expressed in AngⅡ stimulated with 10-5mol / L. Ang Ⅱ induced by 10-7, 10-6 and 10-5mol / L significantly increased the mRNA expression of FN and FN induced by mesangial cells, which was positively correlated with the concentration of AngⅡ. CONCLUSIONS: AngⅡ stimulated the expression of AT1 receptor gene and FN in mesangial cells in a dose-dependent manner, while the high concentration of AngⅡ could up-regulate AT2 receptor expression