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分别以氨基脲(SEM)和1-氨基乙内酰脲(AHD)为模板分子,α-甲基丙烯酸(MAA)为功能单体,乙二醇二甲基丙烯酸酯(EDMA)为交联剂,采用本体聚合方式合成分子印迹聚合物(MIP),建立了分子印迹固相萃取-液相色谱法检测食品中硝基呋喃类药物残留的方法。将MIP制成固相萃取小柱用于样品的前处理过程,并用反相高效液相色谱测定样品中的硝基呋喃类抗生素药物残留。结果表明,SEM和AHD的线性范围分别为1~200μg/g和0.5~200μg/g,线性相关系数分别为0.9991和0.9987。在1.0,5.0,50.0μg/g 3个添加水平下,这两种代谢物的平均回收率在81.0%~85.5%之间,相对标准偏差(RSD)在1.98%~5.01%(n=6)。该方法简便快速、准确可行。
Using semicarbazide (SEM) and 1-aminohydantoin (AHD) as template molecules, α-methacrylic acid (MAA) as functional monomer and ethylene glycol dimethacrylate (EDMA) , A molecularly imprinted polymer (MIP) was synthesized by bulk polymerization. A method for the determination of nitrofuran residues in food by molecularly imprinted solid phase extraction-liquid chromatography was established. The MIP was used as a solid-phase extraction cartridge for sample pretreatment, and the residual nitrobenzamide drug residues in the sample were determined by reverse-phase high performance liquid chromatography. The results showed that the linear range of SEM and AHD were 1 ~ 200μg / g and 0.5 ~ 200μg / g respectively, and the linear correlation coefficients were 0.9991 and 0.9987 respectively. The average recoveries of these two metabolites ranged from 81.0% to 85.5% with relative standard deviations (RSDs) ranging from 1.98% to 5.01% (n = 6) at 1.0, 5.0 and 50.0 μg / . The method is simple and fast, accurate and feasible.