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目的评价脊髓胶质细胞在树酯毒素(RTX)诱导的神经病理性疼痛大鼠的作用。方法雄性健康SD大鼠60只随机均分为两组:RTX组单次腹腔注射RTX 210μg/kg建立神经病理性疼痛模型;C组注射等量溶媒(10%Tween 80、10%乙醇与生理盐水组成的混合液)作为对照。检测两组大鼠建模前及建模后1、3、7、42d机械痛阈值(PWMT)及热痛阈值(PWTL)。分别采用qRT-PCR和Western blot在建模后4h及1、3、7、42d检测CD11b及胶质纤维酸性蛋白(GFAP)mRNA和蛋白表达水平。结果与建模前相比,RTX组建模后3、7、42d大鼠PWMT下降和PWTL升高(P<0.05)。与C组相比,RTX组CD11bmRNA及蛋白表达水平在建模后4h升高(P<0.05),GFAP mRNA及蛋白表达水平在建模后4h及1、3、7、42d升高(P<0.05)。RTX组GFAP表达与PWMT呈负相关(r=-0.96,P<0.01)。结论激活的神经胶质细胞可能在神经病理性疼痛的产生与维持阶段起重要作用。
Objective To evaluate the role of spinal cord glia cells in resin-induced neuropathic pain rats (RTX). Methods Sixty male SD rats were randomly divided into two groups: RTX group was injected intraperitoneally with 210μg / kg of RTX to establish the model of neuropathic pain; Group C was injected with equal volume of medium (10% Tween 80, 10% ethanol and normal saline As a control). The mechanical pain threshold (PWMT) and thermal pain threshold (PWTL) were measured before and at 1, 3, 7 and 42 days after modeling. QRT-PCR and Western blot were used to detect the expression of CD11b and glial fibrillary acidic protein (GFAP) mRNA and protein at 4h, 1,3,7,42d after modeling. Results Compared with those before modeling, the decrease of PWMT and the increase of PWTL in rats in RTX group at 3, 7, and 42 days after modeling (P <0.05). Compared with group C, the expression of CD11b mRNA and protein in RTX group increased at 4h after modeling (P <0.05), while the expression of GFAP mRNA and protein increased at 4h, 1,3,7 and 42 d after modeling (P < 0.05). GFAP expression in RTX group was negatively correlated with PWMT (r = -0.96, P <0.01). Conclusion Activated glial cells may play an important role in the generation and maintenance of neuropathic pain.