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目的探讨外源性Caveolin-1基因在人胃腺癌细胞中的表达作用。方法将pcl-neo-caveolin-1人全长质粒转染入MGC803细胞中,用G418筛选得到细胞克隆并扩大培养,获得稳定表达caveolin-1的细胞系,通过Western-blot、细胞计数及流式细胞术等方法检测基因表达、细胞增殖分化等情况。结果与对照组和pcl-neo空质粒转染组相比,转染caveolin-1基因后的MGC803细胞的caveolin-1的表达明显增高,而P-ERK1/2的表达却明显下降;细胞的群体倍增时间明显延长:由46.67或47.32小时延长至65.46小时,差异有显著性(P<0.01);细胞周期分布发生明显变化:G0/G1期细胞比例由35.02%增加到51.98%,S期比例由56.97%下降至39.80%,G2、M期无明显变化。结论Caveolin-1通过抑制MGC803细胞的增殖而导致其发生G0/G1期阻滞,可为肿瘤基因治疗提供实验依据。
Objective To investigate the expression of exogenous Caveolin-1 gene in human gastric adenocarcinoma cells. Methods The full-length pcl-neo-caveolin-1 plasmid was transfected into MGC803 cells. The cells were screened by G418 and expanded. The cell lines stably expressing caveolin-1 were obtained. Western-blot, cell counting and flow cytometry Cytometry and other methods to detect gene expression, cell proliferation and differentiation. Results The caveolin-1 expression in MGC803 cells transfected with caveolin-1 gene was significantly increased, while the expression of P-ERK1 / 2 was significantly decreased compared with the control and pcl-neo empty plasmid transfected groups. The population of cells The doubling time was significantly prolonged: from 46.67 or 47.32 hours to 65.46 hours, the difference was significant (P <0.01); the cell cycle distribution changed obviously: the proportion of cells in G0 / G1 phase increased from 35.02% to 51.98% 56.97% down to 39.80%, G2, M phase no significant change. Conclusion Caveolin-1 inhibits the proliferation of MGC803 cells and causes G0 / G1 phase arrest, which may provide an experimental basis for gene therapy of cancer.