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应用DNA解旋荧光测定(FADU)法,研究了小剂量辐射对淋巴细胞DNA的影响及其诱导的适应性反应。结果表明,FADU法测得的γ射线所致的淋巴细胞DNA断裂与剂量 呈线性关系,最小检出剂量为0.3Gy;0.5～8.0cGy γ射线对静止期和丝裂原激活后的淋巴细胞双链DNA百分数无影响;小剂量辐射(2.0 cGy)对15Gy γ射线照射所引起的DNA断裂的修复(37℃,15～60min)有促进作用,但对最终修复程度(37℃,120min)无明显提高。小剂量(0.5～4.0 cGy)γ射线照射,均可诱导出淋巴细胞对15 Gyγ射线所致损伤的抗性,以2.0,4.0 cGy的γ射线为诱导适应性反应的最适剂量;大剂量(5～20 Gy)的照射均可显现出小剂量(2.0 cGy)诱导的适应性反应,以15 Gy照射后适应性反应表现最强;3-AB可明显地抑制小剂量辐射诱导的适应性反应。
The effect of low dose radiation on the DNA of lymphocytes and the induced adaptive response were studied by DNA deconvolution fluorescence assay (FADU). The results showed that there was a linear relationship between DNA fragmentation of lymphocytes induced by γ-rays and the dose of FADU, the minimum detectable dose was 0.3Gy; 0.5 ~ 8.0cGy γ-rays had no effect on the number of lymphocytes in resting and mitogen-activated lymphocytes The small dose of radiation (2.0 cGy) promoted the DNA fragmentation (37 ℃, 15 ~ 60min) induced by 15Gy γ-ray irradiation, but had no effect on the final repair degree (37 ℃, 120min) improve. Small doses (0.5 ~ 4.0 cGy) of γ-ray irradiation, can induce lymphocyte injury to 15 Gy γ-ray resistance, with 2.0,4.0 cGy γ-ray as the most appropriate dose to induce adaptive response; high dose 5 ~ 20 Gy) showed a small dose (2.0 cGy) -induced adaptive response, 15 Gy irradiation showed the strongest adaptive response; 3-AB can significantly inhibit the low dose radiation-induced adaptive response .