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研究大肠杆菌表达重组人粒细胞集落刺激；因子（ｒｈＧ－ＣＳＦ）工程菌ＤＨ５α的发酵工艺。方法：采用发酵罐发酵，对影响工程菌生长和目标蛋白的表达条件如：工程菌发酵的培养基配方、ｐＨ值、诱导时间、分批补加营养物质等进行了优化。结果：根据优化的条件，２０Ｌ罐发酵工程菌，菌体收得量可达湿重１５．６ｇ／Ｌ。目标蛋白表达量约占菌体总蛋白的３０％。结论：此发酵工艺可以提高ｒｈＧ－ＣＳＦ工程菌菌体的得率和目标蛋白的表达。
To study the fermentation process of recombinant E. coli expressing human granulocyte colony-stimulating factor (rhG-CSF) engineering strain DH5α. Methods: Fermentation tank fermentation was used to optimize the conditions that affect the growth of engineered bacteria and the expression of the target protein, such as the fermentation medium, the pH value, the induction time, and the supplemental nutrients in batches. Results: According to the optimized conditions, 20L cans of fermentation engineering bacteria, bacteria yield up to 15.6g / L wet weight. The target protein expression accounted for about 30% of the total bacterial protein. Conclusion: This fermentation process can improve the rhG-CSF engineered bacteria yield and target protein expression.