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目的建立清脑宣窍方有效部位中人参皂苷Rg1、Rb1及三七皂苷R1的HPLC含量测定方法。方法采用Phenomenex Luna NH2色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-水(80∶20),流速为1.0 mL/m in,检测波长为203 nm。结果人参皂苷Rg1平均回收率为101.86%,RSD为1.68%(n=9);三七皂苷R1平均回收率为101.23%,RSD为1.28%(n=9);人参皂苷Rb1平均回收率为102.02%,RSD为1.45%(n=9)。结论该方法简便、准确,可用于清脑宣窍方有效部位中人参皂苷Rg1、Rb1及三七皂苷R13种成分的含量测定。
Objective To establish a HPLC method for determination of ginsenosides Rg1, Rb1 and notoginsenoside R1 in the effective fraction of Qingnao Xuanxiong. Methods Phenomenex Luna NH2 column (250 mm×4.6 mm, 5 μm) was used. The mobile phase was acetonitrile-water (80∶20). The flow rate was 1.0 mL/min. The detection wavelength was 203 nm. Results The average recovery of ginsenoside Rg1 was 101.86% with RSD of 1.68% (n=9). The average recovery of sanguinosaide R1 was 101.23%, RSD was 1.28% (n=9), and the average recovery of ginsenoside Rb1 was 102.02. %, RSD is 1.45% (n=9). Conclusion The method is simple and accurate and can be used to determine the contents of ginsenosides Rg1, Rb1 and notoginsenoside R13 in the effective fraction of Qingnao Xuanxiong.