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目的：以大鼠肺Ⅱ型细胞为靶细胞，检测烹调油烟的细胞毒作用和对细胞ＤＮＡ的损伤。方法：烹调油烟颗粒提取物对大鼠肺Ⅱ型细胞染毒２４ｈ后，应用ＭＴＴ法、溴乙锭荧光法以及单细胞凝胶电泳技术检测烹调油烟对大鼠肺Ⅱ型细胞的细胞毒性和ＤＮＡ的损伤。结果：在受试剂量范围内，烹调油烟颗粒提取物具有细胞毒性，可致ＤＮＡ交联及断裂的作用随染毒剂量的增加而增强，并存在剂量－反应关系（ｒ细胞毒性＝－０．９４８，Ｐ＜０．０１；ｒ交联＝０．９７６，Ｐ＜０．０１；ｒ断裂＝０．９５７，Ｐ＜０．０１）。结论：烹调油烟颗粒提取物对大鼠肺Ⅱ型细胞具有细胞毒性作用和ＤＮＡ损伤作用，可使ＤＮＡ链间交联和ＤＮＡ单链断裂。
OBJECTIVE: To detect the cytotoxicity and DNA damage of cooking oil fume using rat lung type Ⅱ cells as target cells. Methods: After exposure to smoke fucoidi extract for 24 hours, the cytotoxicity of cooking oil fume to rat type Ⅱ cells was detected by MTT assay, ethidium bromide assay and single cell gel electrophoresis. Damage. Results: In the range of doses tested, the extract of cooking soot particles was cytotoxic and the DNA cross-linking and cleavage could be enhanced with dose increasing. There was a dose-response relationship (r cytotoxicity -0.0). 948, P <0.01; r cross-link = 0.976, P <0.01; r break = 0.957, P <0.01). CONCLUSION: The cooking oil extract has cytotoxicity and DNA damage on rat lung type Ⅱ cells, which can cross-link DNA and single-stranded DNA.