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为了解河南省乙型肝炎(乙肝)HBV基因型分布及其主蛋白抗原主要亲水区(MHR)氨基酸(aa)位点变异情况。本研究采集河南省2012年HBV流行病学调查的部分HBsAg和HBeAg阳性的血清样本,提取HBV DNA并进行序列扩增,测序得到s基因序列,利用Mega6.0软件比较分析。共得到HBVS基因序列50条,基因型分布B型为16.0%(8/50)、C型为84.0%(42/50)。血清型分布中,adrq+为HBV主要流行血清型,流行率为84.0%。S基因MHR aa位点变异中,T126变异率最高,为14.0%。HBV MHR变异株总流行率为24.0%(12/50),其中B型的突变率为37.5%(3/8),C型的突变率为21.4%(9/42)。河南省乙肝基因型分布以C型为主,B型次之。血清型主要为adrq+为主,adw2次之。HBV MHR aa位点存在变异,应在今后的计划免疫和HBIG治疗中给予重视。
In order to understand the distribution of hepatitis B virus (HBV) genotypes and the variation of amino acid (aa) sites in major hydrophilic regions (MHRs) of hepatitis B virus (HBV) in Henan province. In this study, some HBsAg and HBeAg positive serum samples from epidemiological investigation of HBV in Henan Province in 2012 were collected, HBV DNA was extracted and sequenced. The sequence of s gene was obtained by sequencing and analyzed by Mega6.0 software. A total of 50 HBVS gene sequences were obtained. The genotype distribution was 16.0% (8/50) for B genotype and 84.0% (42/50) for C genotype. Serotype distribution, adrq + is the main epidemic of HBV serotypes, the prevalence was 84.0%. In the mutation of MHR aa gene of S gene, the mutation rate of T126 was the highest (14.0%). The overall prevalence of HBV MHR mutation was 24.0% (12/50). The mutation rate of type B was 37.5% (3/8) and that of type C was 21.4% (9/42). Hepatitis B genotypes in Henan Province are mainly C type, followed by B type. Serotypes mainly adrq +, followed by adw2. HBV MHR aa site variation, should be given in the future planning of immunization and HBIG treatment attention.