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作者选择秘鲁利马郊区社会经济情况较差的800名两月龄婴幼儿作为免疫对象,用双盲法分成4个组,分别口服10~4空斑形成单位(PFU)的G3型恒河猴轮状病毒(RRV)疫苗、G1型人-RRV(D株)重组疫苗,G2型人-RRV(DS1株)重组疫苗和安慰剂.每天两次监测免疫后不良反应,共一周,同时收集免疫后4～5天内大便样品或直肠拭子检测疫苗病毒的排泄情况,大便中的轮状病毒(RV)用G型单克隆抗体ELISA分型检定,不能定型的经培养后重新定型,排泄的疫苗病毒在MA104细胞中培养后用ELISA加以证实.对发生轮状病毒腹泻和随机收集的无腹泻者血清用人血清G1型(Wa株)、G2型(DS1株)石3型(P株)作蚀斑减少中和试验(PRN)分析,被调查的800名儿童中782名被分成1371个儿童年,分别进行了30天以上和两年的连续观察.
The author chose 800 two-month-old infants and young children with poor social and economic conditions in the suburbs of Lima, Peru, as immunization targets and divided them into 4 groups by double-blind method. Each group received 10-4 plaque-forming units (PFU) Rotavirus (RRV) vaccine, recombinant human G1-RRV (strain D) recombinant, recombinant human G2-RRV (DS1) recombinant vaccine and placebo. Adverse reactions were monitored twice daily for one week while collecting the immunizations Stool samples or rectal swabs were tested for the excretion of the vaccine virus within 4 to 5 days after the test, and rotavirus (RV) in the stools were assayed by G-type monoclonal antibody ELISA typing, undefined, cultured, reshaped, and excreted vaccine Viruses were confirmed by ELISA after culturing in MA104 cells.Serum type G1 (Wa strain) and type G2 (DS1 strain) Stones type 3 (strain P) were tested for eradication of rotavirus diarrhea and randomly collected sera without diarrhea The plaque reduction neutralization test (PRN) analysis showed that 782 out of 800 children surveyed were divided into 1371 childhood years and were followed for more than 30 days and two years respectively.