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为研究缺锌及高锌饲料影响大鼠脑发育和学习记忆的机理，选择断乳１～２天健康雄性Ｗｉｓｔａｒ大鼠，建立缺锌组（ＺＤ）、高锌组（ＺＨ）、对照组（ＡＬ）模型。采用原子分光光度计火焰法测定血清和脑锌、铜、铁含量，用ＨＰＬＣ电化学法测定脑组织单胺类递质的含量。结果表明：缺锌及高锌饲料影响大鼠生长发育、食欲和活动状况；ＺＤ组血清和脑锌、铁含量低于ＡＬ组，而脑铜含量高于ＡＬ组，ＺＨ组则血清锌明显高于ＡＬ组，而血清和脑铜、铁含量均明显低于ＡＬ组；ＺＤ组脑组织５ＴＨ及ＺＨ组脑ＮＥ和５ＴＨ明显低于ＡＬ组，而ＺＤ及ＺＨ组５ＨＩＡＡ则明显高于ＡＬ组。提示，缺锌与高锌时大鼠脑组织单胺类递质的紊乱及血清和脑组织锌、铜、铁水平的异常可能是大鼠脑发育和学习记忆障碍的生化基础之一。
To study the mechanism of zinc deficiency and high zinc diets on brain development and learning and memory in rats, we selected healthy male Wistar rats weaned for 1 to 2 days to establish ZD, ZH and control groups AL) model. The contents of zinc, copper and iron in serum and brain were determined by atomic spectrophotometer flame method. The levels of monoamine neurotransmitters in brain tissue were determined by HPLC-electrochemical method. The results showed that zinc deficiency and high-zinc diet influenced the growth, appetite and activity of ZGF rats. ZD rats had lower levels of serum zinc, iron and zinc than those in AL group, In AL group, serum and brain copper and iron contents were significantly lower than those in AL group. In brain tissues of ZD group, NE and 5TH of brain in 5TH and ZH groups were significantly lower than those in AL group, while those of ZD and ZH group were significantly higher In AL group. It is suggested that the disorder of monoamine neurotransmitters and the abnormal levels of zinc, copper and iron in serum and brain tissue may be one of the biochemical basis of rat brain development and learning and memory impairment.