对十种牧草花粉进行超低温贮存,并对其贮存技术进行研究。在超低温(即 LN_2中,-196℃)的条件下,贮存一年,各种牧草花粉活力均有不同程度的下降,各种类间差异较大。同一种牧草花粉,在 LN_2中贮存一个月、三个月、一年,花粉活力下降呈递增趋势。大多数牧草花粉在超低温贮存过程中,加冷冻保护剂效果反而不好。冷冻前控制花粉水份含量是保持其存活率的重要因素。对花粉活力的检测,采用过氧化物酶测定法准确且迅速。 Ten species of grass pollen were cryopreserved and their storage techniques were studied. Under ultra-low temperature (ie, LN_2, -196 ℃) conditions, after one year of storage, the vigor of the pollen of all kinds of grasses decreased with different degrees, and the differences among different species were relatively large. The same kind of grass pollen, stored in LN_2 for one month, three months, one year, decreased pollen activity showed an increasing trend. Most forage pollen in the ultra-low temperature storage process, plus the cryoprotectant effect is not good. Control of pollen moisture content before freezing is an important factor to maintain its survival rate. Pollen vitality of the test, using peroxidase assay accurate and rapid.