转基因种子快速准确筛查对于保障农业转基因生物安全管理非常重要。作者将DNA热碱提取法和复合荧光PCR技术相结合,建立起玉米种子转基因成分快速筛查方法。结果显示,该方法不仅能保证检测的准确性和灵敏性,而且还大大降低假阳性发生率,在进行大批量玉米种子样品的转基因成分快速检测时,极大地提高了转基因筛查的效率。 Rapid and accurate screening of genetically modified seeds is very important for the safe management of agricultural genetically modified organisms. The authors combined the DNA hot-alkali extraction method and the complex fluorescent PCR technique to establish a rapid screening method for the transgenic components of corn seeds. The results show that this method can not only ensure the accuracy and sensitivity of the detection, but also greatly reduce the incidence of false positives and greatly improve the efficiency of transgenic screening in the rapid detection of the transgenic components of large-volume corn seed samples.