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目的探讨低压低氧环境下帕金森病(PD)模型小鼠学习记忆能力的变化。方法 60只SPF级雄性昆明小鼠,体质量17~22 g。随机分为1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)组(PD模型组)、低压低氧(HH)组、MTPT+HH组和对照组,每组15只。以动物实验用高原环境模拟舱模拟低压低氧环境,通过腹腔注射MPTP诱导PD模型小鼠,使用Y型-电迷宫和跳台实验评价小鼠学习记忆能力。结果与对照组比较,MPTP组、HH组和MPTP+HH组Y型-电迷宫学习和记忆总反应时间(TRT)明显延长(P均<0.01),错误反应次数(EN)明显增多(P均<0.01);跳台实验学习和记忆TRT明显延长(P均<0.01),EN明显增多(P均<0.01)。且上述各指标MPTP+HH组分别低于MPTP组与HH组(P均<0.05),而MPTP组与HH组之间水平相近(P均>0.05)。结论低压低氧加重了MPTP所致PD模型小鼠学习记忆能力的下降程度。
Objective To investigate the changes of learning and memory ability in mice with Parkinson’s disease (PD) under hypobaric hypoxia. Methods Sixty SPF male Kunming mice, body weight 17 ~ 22 g. The rats were randomly divided into 4 groups: MPTP group, PDH group, MTPT + HH group and control group, 15 in each group Animals were experimentally simulated hypobaric hypoxia environment using plateau environment simulation cabin. PD mice were induced by intraperitoneal injection of MPTP, and the learning and memory abilities of mice were evaluated by Y-type electric maze and platform jumping test. Results Compared with the control group, the Y-type electrical maze and the total memory time (TRT) in MPTP group, HH group and MPTP + HH group were significantly prolonged (P <0.01) and the number of false reaction significantly increased <0.01). The TRT of platform learning and memory was significantly prolonged (P <0.01) and EN significantly increased (P <0.01). The indexes of MPTP + HH group were lower than those of MPTP group and HH group (all P <0.05), while those of MPTP group and HH group were similar (all P> 0.05). Conclusions Hypobaric hypoxia aggravates the decrease of learning and memory abilities in PD model mice induced by MPTP.