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目的观察L-精氨酸对生长受限孕鼠胎盘胰岛素样生长因子-Ⅰ(IGF-Ⅰ)及其受体(IGF-IR)表达的影响,探讨L-精氨酸对生长受限孕鼠的作用机制。方法取健康SD系大鼠雌雄3:1合笼饲养,按妊娠顺序随机抽取孕鼠分为空白组、模型组、观察组(L-精氨酸组)各12只,模型组、观察组孕8 d至孕20 d采用被动吸烟造模,模型组孕鼠腹腔注射生理盐水,观察组孕鼠腹腔注射L-精氨酸液,孕21 d剖宫取胎,计数活胎数,测量活胎仔身长、体质量、头臀长及胎盘质量并制备胎盘标本,采用免疫组织化学法检测IGF-I及IGF-IR。结果模型组胎鼠体质量、头臀长、胎盘质量均低于空白组与观察组(P<0.05),而观察组胎鼠体质量、头臀长、胎盘质量与空白组比较无明显差别(P>0.05)。光镜下各组胎盘均见IGF-I免疫反应阳性,模型组IGF-I、IGF-IR均低于空白组与观察组(P<0.05),而观察组IGF-I、IGF-IR与空白组比较无明显差别(P>0.05)。结论L-精氨酸可能通过直接或间接促进IGF-I的合成和分泌,促进吸烟所致生长受限胎仔的生长发育。
Objective To investigate the effect of L-arginine on the expression of insulin-like growth factor-Ⅰ (IGF-Ⅰ) and its receptor (IGF-Ⅰ) in placenta of pregnant rats with limited growth and to investigate the effect of L-arginine on the growth- The mechanism of action. Methods Male and female SD rats were housed in a 3: 1 cage at random. Pregnant rats were randomly divided into blank group, model group and observation group (L-arginine group), 12 in each model group, observation group The rats in model group were intraperitoneally injected with saline at 8 d to 20 d of pregnancy. L-arginine solution was injected intraperitoneally into the pregnant rats in the model group, and the cesarean section was taken at 21 d of pregnancy. The number of live fetuses was counted and the number of live fetuses Body length, body weight, head and hip length, placenta quality and placenta preparation were measured. Immunohistochemistry was used to detect IGF-I and IGF-IR. Results The body weight, head and buttock length and placenta quality of the model group were lower than those of the blank group and the observation group (P <0.05). There was no significant difference between the control group and the blank group (P <0.05) P> 0.05). IGF-I and IGF-IR in model group were lower than those in blank group and observation group (P <0.05), while IGF-I and IGF-IR in observation group were significantly higher than blank group There was no significant difference between the two groups (P> 0.05). Conclusion L-arginine may promote the growth and development of fetal growth-restricted fetuses by smoking directly or indirectly by promoting the synthesis and secretion of IGF-I.