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目的:探讨Fos与磷酸化的转录因子环磷酸腺苷反应元件结合蛋白(cAMP responsive element binding protein,CREB)在正常昆明小鼠前脑的表达及相互关系。方法:成年昆明小鼠,4%多聚甲醛灌注固定、取脑、冰冻切片30μm厚,应用免疫组化法检测Fos和磷酸化CREB(phosphorylated CREB,pCREB)的表达。结果:(1)前脑Fos与pCREB表达较高且一致的脑区主要在前扣带回皮质、顶皮质、梨状前皮质、扣带后回和压部皮质等;(2)二者表达一致但较低的脑区,包括海马CA1、CA3区、隔内侧核、苍白球、梨状内核和杏仁基外侧核等。结论:在生理条件下,Fos在边缘系统的较高表达与边缘系统功能调节有关,pCREB在边缘系统的较高表达与生理性刺激的诱导有关,也与边缘系统的调节有关,二者在前脑的表达分布表明CREB的磷酸化启动了Fos的表达。
AIM: To investigate the expression and correlation of Fos and phosphorylated transcription factor cAMP responsive element binding protein (CREB) in the forebrain of normal mice. METHODS: Adult Kunming mice were perfused with 4% paraformaldehyde. The sections of brain and frozen section were harvested and the thickness of 30μm was determined. The expressions of Fos and phosphorylated CREB (pCREB) were detected by immunohistochemistry. Results: (1) The forebrain Fos and pCREB expressed higher and consistent brain regions mainly in the anterior cingulate cortex, parietal cortex, pre-piriform cortex, cingulum back and pressure cortex, etc .; (2) the expression of both Uniform but low brain regions, including hippocampal CA1, CA3 area, the medial septum, globus pallidus, pear-shaped nucleus and almond-based lateral nucleus. Conclusion: Under physiological conditions, the higher expression of Fos in limbic system is related to the regulation of limbic system. The higher expression of pCREB in limbic system is related to the induction of physiological stimuli and the regulation of limbic system. Brain expression profiles indicate that CREB phosphorylation initiates Fos expression.