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１５只ＳＤ大鼠随机分为氰戊菊酯（Ｆｅｎ）组（１２０ｍｇ·ｋｇ－１·ｄ－１×７，ｉｇ），苯巴比妥（ＰＢ）诱导组（８０ｍｇ·ｋｇ－１·ｄ－１×３，ｉｐ）及溶剂对照组（给予Ｆｅｎ组等体积的二甲亚砜，处理同Ｆｅｎ组）．应用不连续ＳＤＳ－ＰＡＧＥ电泳分离细胞色素Ｐ４５０，用ＣＯ差示光谱法和细胞色素Ｃ还原法测定Ｐ４５０含量及ＮＡＤＰＨ－细胞色素Ｐ４５０还原酶活性，用ＣＹＰ２Ｂ１／２Ｂ２抗体及ＣＹＰ３Ａ１／３Ａ２抗体进行Ｗｅｓｔｅｒｎ印迹法分析，用免疫组化和半定量ＲＴ－ＰＣＲ等技术观察Ｆｅｎ影响大鼠脑，肝组织细胞色素Ｐ４５０亚型的特异性．结果显示，与溶剂对照组相比Ｆｅｎ可使脑和肝中Ｐ４５０含量及ＮＡＤＰＨ－Ｐ４５０还原酶活性增高；Ｐ４５０２Ｂ１／２Ｂ２增加，Ｐ４５０３Ａ１／３Ａ２未见改变；Ｐ４５０２Ｂ１／２Ｂ２ｍＲＮＡ含量相应增加．由于正常大鼠脑，肝组织中Ｐ４５０２Ｂ１／２Ｂ２表达很低，Ｆｅｎ对该亚型Ｐ４５０的诱导可能会干扰机体对内，外源化合物的正常代谢，从而产生毒作用．
Fifteen SD rats were randomly divided into Fen group (120mg · kg-1 · d-1 × 7, ig), phenobarbital (80mg · kg-1 · d- 1 × 3, ip) and solvent control group (given Fen group equal volume dimethyl sulfoxide treatment with the Fen group). Cytochrome P450 was separated by discontinuous SDS-PAGE electrophoresis. P450 content and NADPH-cytochrome P450 reductase activity were measured by CO differential spectroscopy and cytochrome C reduction assay. Western blotting was performed with CYP2B1 / 2B2 antibody and CYP3A1 / 3A2 antibody Method analysis, immunohistochemistry and semi-quantitative RT-PCR technology Fen observed rat brain, liver cytochrome P450 subtype specificity. The results showed that Fen could increase P450 content and NADPH-P450 reductase activity in brain and liver compared with solvent control group; P4502B1 / 2B2 increased but P4503A1 / 3A2 had no change; P4502B1 / 2B2 mRNA increased accordingly. Due to the low expression of P4502B1 / 2B2 in normal rat brain and liver tissue, the induction of this subtype P450 by Fen may interfere with the normal metabolism of exogenous and endogenous compounds in the body and thus produce toxic effects.