论文部分内容阅读
[目的]采用高效液相色谱法,建立砜吡草唑在土壤与水中残留的分析方法,为砜吡草唑的检测技术提供技术支撑。[方法]使用Symmetry TM RP18柱,以乙腈-水(体积比60∶40)为流动相,在UV 226 nm下,用外标法对有效成分砜吡草唑进行定量分析。[结果]该分析方法的变异系数为0.27%,平均回收率为100.3%,线性相关系数为0.9999。采用丙酮为提取剂,二氯甲烷萃取的方法,提取水中和土壤中的砜吡草唑残留量。水中平均回收率92.5%~107.0%,相对标准偏差为2.9%~3.2%;土壤中平均回收率为76.3%~88.0%,相对标准偏差为0.2%~4.3%。[结论]该方法分析速度快、灵敏度高、重现性好,适用于砜吡草唑的快速检测和确证。
[Objective] The research aimed to establish a method for the determination of sulforapiol in soil and water by high performance liquid chromatography (HPLC), and provide technical support for the detection of sulforazole. [Method] The content of sulfamethoxazole was quantified by UV spectrophotometry with Symmetry TM RP18 column using acetonitrile - water (60:40 by volume) as the mobile phase at UV 226 nm. [Result] The coefficient of variation of this method was 0.27%, the average recovery was 100.3% and the linear correlation coefficient was 0.9999. Acetone was used as extractant and dichloromethane extraction method to extract the residues of sulfamethoxazole in water and soil. The average recoveries in water ranged from 92.5% to 107.0%, with relative standard deviations from 2.9% to 3.2%. The average recoveries in soils ranged from 76.3% to 88.0% with relative standard deviations of 0.2% to 4.3%. [Conclusion] The method was rapid, sensitive and reproducible. It was suitable for the rapid detection and confirmation of sulfamethoxazole.