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目的:探讨长期铝暴露小鼠记忆功能和血清生化指标的变化及何首乌复方制剂对其治疗作用效果。方法:每天用16g/L浓度的(AICl3)氯化铝拌饲料喂养小白鼠,同时用2g/L硝酸铝溶液给小鼠自由饮用。连续5个月(150d)染毒实验。染毒到100d开始将染毒小鼠分组:治疗1组、治疗2组、模型组,另设一正常组(对照组),治疗1组用何首乌复方制剂灌胃治疗,治疗2组用排重金属中药制剂海尔福口服液灌胃治疗作为治疗对照组,模型组和正常组用等体积蒸馏水灌胃作正常对照。100d后治疗与染毒同时进行,直到实验结束。实验前、66、104、125、132、145d进行水迷宫测定记忆力。实验结束,从眼球取血,分离前测定血清生化指标,然后处死动物,取大脑用甲醛处理后作病理检查(另报道)。结果:治疗4d(染毒104d)及之前各组游水逃避潜伏期(s)时间无明显差异,但治疗4d正常组错误率为0,明显低于其他各组;治疗25d时,治疗1组和正常组逃避潜伏期(s)时间和错误率明显低于其他两组;治疗32d和45d模型组逃避潜伏期(s)时间和错误率明显高于其他3组。血清生化:各组间比较P<0.01;TG和尿素各组间尚无明显差异。结论:长期铝暴露所致小鼠有明显的记忆力障碍,血清生化指标有不同程度的变化,治疗后有明显的好转。
Objective: To investigate the changes of memory function and serum biochemical indexes in long-term aluminum exposed mice and the therapeutic effect of the Polygonum multiflorum compound on it. Methods: Mice were fed daily with 16g / L aluminum chloride (AICI3), while mice were drank freely with 2g / L aluminum nitrate solution. Continuous five months (150d) exposure experiment. After exposure to 100d, the exposed mice were divided into two groups: treatment 1 group, treatment 2 group, model group and another normal group (control group). The treatment group 1 was given ganpifloxacin by intragastric administration, As a control group, Hailu Fu oral liquid was given as the control group, and the model group and the normal group were given normal volume of distilled water with equal volume. After 100d treatment and exposure simultaneously, until the end of the experiment. Before the experiment, 66,104,125,132,145 d water maze determination of memory. At the end of the experiment, blood was taken from the eye and serum biochemical parameters were measured before separation. Animals were sacrificed and the brain was treated with formaldehyde for pathological examination (another report). Results: There was no significant difference in swimming escape latency (4 s) between 4d (104d) and before swimming, but the error rate of normal group 4d after treatment was 0, which was significantly lower than that of other groups. On the 25th day of treatment, The escape latency (s) and the error rate of the two groups were significantly lower than those of the other two groups. The escape latency (s) and error rate of the 32d and 45d groups were significantly higher than those of the other three groups. Serum biochemistry: P <0.01 in each group; there was no significant difference between TG and urea groups. CONCLUSION: Mice with long-term exposure to aluminum have obvious memory impairment, serum biochemical indexes have varying degrees of change, and have obvious improvement after treatment.