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作者运用细胞培养、膜片钳和激光共聚焦技术 ,观察了降钙素基因相关肽 (CGRP)对正常及模拟缺血、缺氧心肌细胞钙通道的作用及其电生理离子机制 ,并利用计算机重建技术在心肌细胞通道动力学及电流重建等方面进行了探索性研究 ,得到了如下提示性结论 :1CGRP对心肌的保护作用有赖于适当剂量和浓度 ;2本课题建立的急性心肌细胞分离方法 ,能够得到具有正常电生理特性的耐钙心肌细胞 ,是膜片钳实验和激光扫描共聚焦技术测定胞内钙的基础 ;3CGRP对急性缺血、缺氧心肌细胞具有保护作用 ,在急性缺血、缺氧时 ,急性分离的心肌细胞胞内钙离子浓度降低 ,CGRP可引起胞内钙浓度稍微升高 ,且 CGRP可改善因急性缺血、缺氧引起的胞内钙浓度的降低 ,其机制有待于进一步探讨 ;4CGRP能促使正常及模拟缺血缺氧状态下豚鼠心室肌细胞 ICa内流的增加 ,是 CGRP正性作用的主要离子机制 ;CGRP对钙通道的作用机制还有待进一步研究 ;5本课题建立的对几种离子电流的计算机重建方法 ,提示可以被用来讨论离子电流机制 ,是一种新的途径
Using cell culture, patch-clamp and confocal laser scanning microscopy, we observed the effect of calcitonin gene-related peptide (CGRP) on calcium channel in normal and simulated ischemia and hypoxia cardiomyocytes and its electrophysiological mechanism. Reconstruction techniques in cardiomyocyte channel dynamics and current reconstruction and other aspects of the exploratory study, the following conclusions are drawn: 1CGRP on myocardial protection depends on the appropriate dose and concentration; 2 this project established acute cardiomyocyte separation method, Can get the cardiomyocytes with normal electrophysiological properties, is the basis of patch clamp test and laser scanning confocal technique for determination of intracellular calcium; 3CGRP has a protective effect on acute ischemia, hypoxic cardiomyocytes in acute ischemia, In hypoxia, the intracellular calcium concentration of acutely isolated cardiomyocytes is decreased, CGRP can cause a slight increase of intracellular calcium concentration, and CGRP can improve the reduction of intracellular calcium concentration caused by acute ischemia and hypoxia To further explore; 4CGRP can promote normal and simulated ischemia and hypoxia in guinea pig ventricular myocytes ICa influx, is a positive role of CGRP To ionic mechanisms; of CGRP to be further studied the mechanism of action as well as calcium channel; computer reconstruction of the ion current of several 5 subject established prompt may be used to discuss the mechanism of the ion current, it is a new approach