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目的探讨参附注射液(ShenfuInjection)对大鼠全脑缺血再灌注时核转录因子-κB(nuclear factor kappaB,NF-κB)表达的影响及其治疗作用。方法采用四血管阻塞的方法复制出大鼠全脑缺血再灌注模型,采用免疫组化法、原位杂交法和原位末端标记法分别检测假手术组(A组)、全脑缺血再灌注组(B组)、参附注射液治疗组(C组)海马CA1区NF-κB、肿瘤坏死因子-αmRNA(TNF-αmRNA)表达及细胞凋亡数的变化。结果与假手术组比较,全脑缺血再灌注组海马CA1区NF-κB和TNF-αmRNA的表达及细胞凋亡数明显增加(P<0.01);NF-κB和TNF-αmRNA的表达分别于再灌注6h和12h达到高峰,并持续到48h;细胞凋亡数随再灌注时间的延长而逐渐增多(P<0.01)。参附注射液治疗后NF-κB和TNF-αmRNA的表达下降,细胞凋亡数减少(P<0.01)。结论在脑缺血再灌注救治过程中参附注射液可能通过抑制NF-κB与TNF-αmRNA的表达,进而减少细胞凋亡而发挥脑保护作用。
Objective To investigate the effect of Shenfu Injection on the expression of nuclear factor kappaB (NF-κB) in rats with global cerebral ischemia-reperfusion and its therapeutic effects. Methods The rat model of global cerebral ischemia and reperfusion was duplicated by four-vessel obstruction. Immunohistochemistry, in situ hybridization and in situ end labeling were used to detect sham operation group (A group) and global cerebral ischemia respectively. The changes of NF-κB, tumor necrosis factor-α mRNA (TNF-α mRNA) expression and apoptotic cell number in hippocampal CA1 region in the infusion group (B group) and Shenfu injection group (C group). Results Compared with the sham-operated group, the expression of NF-κB and TNF-α mRNA and the number of apoptotic cells in the hippocampus CA1 region were significantly increased in the global cerebral ischemia-reperfusion group (P<0.01). The expression of NF-κB and TNF-α mRNA was detected in The reperfusion peaked at 6h and 12h and continued for 48h. The number of apoptosis increased with the increase of reperfusion time (P<0.01). After Shenfu injection treatment, the expression of NF-κB and TNF-α mRNA decreased and the number of apoptotic cells decreased (P<0.01). Conclusion Shenfu injection may play a role in brain protection by inhibiting the expression of NF-κB and TNF-α mRNA and decreasing apoptosis during cerebral ischemia-reperfusion.