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目的测定不同产地铁苋菜和绒毛龙芽草中没食子酸的含量,并考察不同提取溶剂对没食子酸含量测定结果的影响。方法采用HPLC法,AttimaC18柱(5μm,4.6mm×250mm),流动相为乙腈-0.1%三乙胺(稀磷酸调pH值至3.5)(1.5∶98.5),流速1.0ml·min-1,检测波长212nm。结果线性回归方程为Y=5.09×104+1.77×106X,r=0.9998,线性范围为0.8304～4.1520μg。6个不同产地药材,以江西产铁苋菜和绒毛龙芽草中没食子酸含量最高,分别为0.545%和0.062%。不同提取溶剂比较,以水为提取溶剂所测铁苋菜和绒毛龙芽草中没食子酸含量最高,分别为0.465%和0.036%。结论不同产地铁苋菜和绒毛龙芽草中没食子酸含量存在差异。
OBJECTIVE To determine the content of gallic acid in different productions of M. avenae and A. virens, and to investigate the effect of different extraction solvents on the determination of gallic acid content. Methods The HPLC method was used on an Attima C18 column (5 μm, 4.6 mm×250 mm). The mobile phase consisted of acetonitrile-0.1% triethylamine (pH of dilute phosphoric acid was adjusted to 3.5) (1.5:98.5) and the flow rate was 1.0 ml·min-1. Wavelength 212 nm. The linear regression equation was Y=5.09×104+1.77×106X, r=0.9998, and the linear range was 0.8304-4.1520 μg. The contents of gallic acid in the six different origins of Chinese medicine were the highest in the iron-containing leek in Jiangxi province and the dragon gall grass in the Jiangxi province, which were 0.545% and 0.062% respectively. Compared with different extraction solvents, the highest content of gallic acid was found in the water-extracting solvent and the contents of gallic acid were 0.465% and 0.036%, respectively. Conclusion There is a difference in gallic acid content in different production of Amaranthus communis and A. viridis.