论文部分内容阅读
目的:观察体外高渗灌注对正常及高血压大鼠心肌缺血及再灌注损伤的影响。方法:利用体外心脏逆行灌注装置将大鼠心脏在予以正常灌注液或高渗透压灌注液灌注后进行缺血及再灌注,监测缺血前后心肌功能、缺血后心肌肌酸激酶释放量,用高效液相加电化学法测定心肌儿茶酚胺释放量,用原子吸收光谱法测定再灌注后心肌钙离子含量。结果:高渗灌注可显著减轻正常及高血压大鼠心肌缺血损害,改善高血压大鼠缺血后心功能,减少心肌儿茶酚胺释放,但未能减少再灌注后心肌钙含量。结论:高渗灌注减轻心肌缺血与再灌注损害,其作用可能与减少心肌儿茶酚胺释放有关。
Objective: To observe the effect of hypertonic perfusion in vitro on myocardial ischemia and reperfusion injury in normal and hypertensive rats. Methods: The heart of rats were perfused with normal perfusion fluid or hyperosmolar perfusion fluid after ischemia reperfusion by using retrograde perfusion device. The myocardial function and myocardial creatine kinase release before and after ischemia were monitored. The release of myocardial catecholamines was determined by high performance liquid electrochemistry and the content of calcium in myocardium was measured by atomic absorption spectrometry. Results: Hypertonic perfusion could significantly reduce myocardial ischemia-reperfusion injury in normal and hypertensive rats, improve cardiac function after ischemia in hypertensive rats and decrease myocardial catecholamine release, but failed to reduce myocardial calcium content after reperfusion. Conclusion: Hypertonic perfusion relieves myocardial ischemia and reperfusion injury, which may be related to the decrease of cardiac catecholamine release.