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检测细胞周期蛋白Ｄ１（ＣｙｃｌｉｎＤ１）在肺癌及癌前病变和正常肺组织的表达，探讨它在肺癌诊断中的作用及其与生物学行为的关系。方法收集肺癌及癌前病变和正常肺组织，采用免疫组化ＳＰ法检测ＣｙｃｈｎＤ１的表达。结果正常肺组织和鳞状化生的支气管上皮未见ＣｙｃｌｉｎＤ１表达，在轻－中－重度不典型增生、原位癌、浸润癌，ＣｙｃｌｉｎＤ１表达的阳性率逐渐升高，阳性细胞数也增多，肺癌组织中ＣｙｃｌｉｎＤ１表达的总阳性率为５６．４％。低分化癌的阳性率高于中高分化癌（Ｐ＜０．０５）：无论鳞癌还是腺癌，ＣｙｃｌｉｎＤ１阳性组的增殖活性高于阴性组（Ｐ＜０．０５）。肺鳞癌有淋巴结转移组ＣｙｃｌｉｎＤ１的阳性率高于无转移组（Ｐ＜０．０５）。结论ＣｙｃｌｉｎＤ１表达与肺癌的发生和增殖有关，而且ＣｙｃｌｉｎＤ１表达是支气管粘膜上皮癌变的早期事件，提示ＣｙＣｌｉｎＤ１可作为判断肿瘤发生可能性的标记物，在肺癌的早期诊断中可能具有重要意义。ＣｙＣｌｉｎＤ１表达与肺鳞癌分化不良及淋巴结转移有关，可能是预后不良的标志。
To detect the expression of CyclinD1 in lung cancer, precancerous lesions and normal lung tissues and to explore its role in the diagnosis of lung cancer and its relationship with biological behavior. Methods Lung cancer, precancerous lesions and normal lung tissues were collected. The expression of CychnD1 was detected by immunohistochemical SP method. Results No expression of CyclinD1 was observed in normal lung tissues and squamous metaplastic bronchial epithelium. The positive rate of the expression of CyclinD1 in carcinoma of in situ, invasive carcinoma and positive cell number increased in patients with mild-moderate-severe dysplasia and lung cancer The total positive rate of CyclinD1 expression in the tissue was 56.4%. The positive rate of poorly differentiated carcinoma was higher than that of moderately differentiated carcinoma (P <0.05). The proliferative activity of CyclinD1 positive group was higher than that of negative group (P <0.05), regardless of squamous cell carcinoma or adenocarcinoma. The positive rate of CyclinD1 in squamous cell carcinoma with lymph node metastasis was higher than that in non-metastasis (P <0.05). Conclusions The expression of CyclinD1 is related to the occurrence and proliferation of lung cancer, and the expression of CyclinD1 is an early event of bronchial epithelial carcinogenesis. It suggests that CyClinD1 may be used as a marker to determine the possibility of tumorigenesis and may be of great significance in the early diagnosis of lung cancer. CyClinD1 expression and lung squamous cell dysplasia and lymph node metastasis, may be a sign of poor prognosis.