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目的 观察口腔鳞癌及口腔粘膜高危型人乳头状瘤病毒 (HPV)感染情况 ,探讨HPV感染与临床及病理资料的关系。方法 用多聚酶链反应 (PCR)检测 73例口腔鳞癌及 40例正常口腔粘膜石蜡包埋组织中HPV16、HPV18的DNA。统计分析其与临床及病理资料的关系。结果 口腔鳞癌HPV16 / 18DNA阳性率为 74% (5 4/ 73) ,正常口腔粘膜为 5 5 % (2 2 / 40 )。口腔鳞癌与正常口腔粘膜HPV阳性率存在显著性差异 (P =0 .0 40 )。统计分析显示 :HPV感染与患者的性别、年龄有关 ,与其它因素 (肿瘤发生部位、嗜烟酒情况、肿瘤病理分级、临床分期 )无关。结论 高危型HPV感染与口腔鳞癌的发生有关。口腔粘膜中HPV感染普遍存在 ,提示HPV在口腔肿瘤的发生中并非独立发挥作用。
Objective To observe the infection of high risk human papilloma virus (HPV) in oral squamous cell carcinoma and oral mucosa and to explore the relationship between HPV infection and clinical and pathological data. Methods The DNA of HPV16 and HPV18 in 73 cases of oral squamous cell carcinoma and 40 cases of normal oral mucosa paraffin embedded tissues were detected by polymerase chain reaction (PCR). Statistical analysis of its relationship with clinical and pathological data. Results The positive rate of HPV16 / 18 DNA in oral squamous cell carcinoma was 74% (54/73) and that in normal oral mucosa was 5 5% (2 2/40). Oral squamous cell carcinoma and normal oral mucosa HPV positive rate there is a significant difference (P = 0.040). Statistical analysis showed that HPV infection was related to the gender and age of the patients and had no relation with other factors (tumor location, alcohol and tobacco consumption, tumor pathological grade and clinical stage). Conclusion High-risk HPV infection is associated with the occurrence of oral squamous cell carcinoma. HPV infection in oral mucosa is widespread, suggesting that HPV does not play an independent role in the occurrence of oral tumors.