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目的:研究骨桥蛋白OPN在胰腺癌中的表达水平及其与临床病理特征的关系。方法:采用RT-PCR和免疫组织化学方法分别检测50例胰腺癌手术切除标本和20例癌旁正常胰腺组织中OPNmRNA及其蛋白水平的表达。结果:胰腺癌组织中OPNmRNA高表达率为90%(45/50)明显高于其在癌旁正常胰腺组织中的表达15%(3/20),差异有统计学意义(P<0.01);OPN蛋白的阳性率为86.0%(43/50),明显高于癌旁正常胰腺组织中的表达30%(6/20),差异有统计学意义(P<0.01);OPN在胰腺癌组织中表达与其淋巴结转移明显相关(P<0.05)。结论:OPN在胰腺癌中的过表达对胰腺癌的生长、浸润及转移起重要作用。
Objective: To study the expression of osteopontin OPN in pancreatic cancer and its relationship with clinicopathological features. Methods: The expression of OPN mRNA and protein in 50 cases of pancreatic cancer and 20 cases of adjacent normal pancreatic tissue were detected by RT-PCR and immunohistochemistry respectively. Results: The high expression rate of OPN mRNA in pancreatic cancer tissues was 90% (45/50), which was significantly higher than that in normal pancreatic tissues (15%, 3/20). The difference was statistically significant (P <0.01). The positive rate of OPN protein was 86.0% (43/50), which was significantly higher than that in the adjacent normal pancreatic tissue (30%, 6/20), the difference was statistically significant (P <0.01). The positive rate of OPN protein in pancreatic cancer tissues Expression was significantly associated with lymph node metastasis (P <0.05). Conclusion: Overexpression of OPN in pancreatic cancer plays an important role in the growth, invasion and metastasis of pancreatic cancer.