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利用放射免疫分析法测定了排卵、排精前后青岛文昌鱼（Ｂｒａｎｃｈｉｏｓｔｏｍａ ｂｅｌｃｈｅｒｉ）体内促性腺激素释放激素（ＧｎＲＨ）的含量变化，并通过高效液相色谱比较了雌、雄文昌鱼性腺及除性腺外体部ＧｎＲＨ的种类和含量的异同。结果表明：ｌ）生殖过程中雌、雄文昌鱼体内ＧｎＲＨ含量的变化规律不同；雌性文昌鱼体内ＧｎＲＨ总含量在排卵时有所下降，排后１２小时下降最为明显，以后逐渐上升到排前水平；雄性文昌鱼仅在排精时有所下降，２小时后即稳定在排前水平。２）文昌鱼卵巢和精巢中均存在较高含量的哺乳类ＧｎＲＨ（ｍＧｎＲＨ）和较低含量的鱼类ＧｎＲＨ（ｓＧｎＲＨ），并且两种ＧｎＲＨ的含量在卵巢和精巢中无明显差异。３）雌、雄文昌鱼体部的ｍＧｎＲＨ和ｓＧｎＲＨ含量存在较大差异：雌性中以ｍＧｎＲＨ为主，而雄性中ｓＧｎＲＨ的含量则远高于雌性中的含量。本工作同时提示在性腺发育及生殖过程中文昌鱼体内的ｍＧｎＲＨ可能较ｓＧｎＲＨ起更重要的作用。
Radioimmunoassay was used to determine the content of gonadotropin-releasing hormone (GnRH) in the ovaries and the gonads of Branchiostoma belcheri before and after ovulation. The gonadotrophin (GnRH) The similarities and differences between the types and contents of GnRH in the body. The results showed that: (1) The variation of GnRH content in female and male amphioxus was different during the reproductive process. The total content of GnRH in female amphioxus decreased during ovulation and decreased most significantly after 12 hours of discharge, ; Male amphioxus decreased only during the time of ejaculation and stabilized at the top of the order two hours later. 2) There was higher content of GnRH (mGnRH) and lower level of GnRH (sGnRH) in both ovary and testis of amphioxus, and the content of GnRH in ovary and testis showed no significant difference. 3) The contents of mGnRH and sGnRH in the body of male and female amphioxus are quite different: mGnRH is predominant in female, while the content of sGnRH in male is much higher than that in female. This work also suggests that mGnRH in amphioxus may play a more important role than sGnRH during gonadal development and reproduction.