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目的:探讨Toll样受体2(TLR2)、Toll样受体4(TLR4)、髓样分化因子88(MyD88)在实验性慢性末端回肠炎(CTI)发病过程中表达水平的变化及其相关意义。方法:60只雄性清洁级SD大鼠随机分成造模组、缝线组、对照组。分别利用肉眼及镜下观察、Western Blot方法,分析3种因子在CTI发病中的变化及其相关性。结果:(1)术后2周及8周,肉眼及镜下观见造模组表现分别为急性及慢性炎症反应。(2)术后8周与2周对比,结果表明TLR2、TLR4、MyD88在末端回肠炎的发病过程中有明显的升高。TLR4表达水平的升高率更显著。(3)在实验性末端回肠炎发病过程中TLR2、TLR4、MyD88表达呈正相关。结论:(1)TLR2、TLR4、MyD88在CTI中表达增加,均与组织炎症程度呈正相关;(2)TLR4表达水平在CTI发病时较TLR2及MyD88升高更显著,推测革兰阴性菌感染可能是CTI发病的启动因子。
Objective: To investigate the expression of TLR2, TLR4 and MyD88 in the pathogenesis of experimental chronic terminal ileitis (CTI) and its significance . Methods: Sixty male SD rats were randomly divided into model group, suture group and control group. Macroscopic and microscopic observation, Western Blot method were used to analyze the changes of three kinds of factors in the pathogenesis of CTI and its correlation. Results: (1) After 2 weeks and 8 weeks, the macroscopic and microscopic findings of the model group showed acute and chronic inflammatory reactions respectively. (2) After 8 weeks and 2 weeks, the results showed that TLR2, TLR4, MyD88 in the pathogenesis of terminal ileitis significantly increased. The rate of TLR4 expression increased more significantly. (3) There was a positive correlation between TLR2, TLR4 and MyD88 in the pathogenesis of experimental terminal ileitis. Conclusions: (1) The expressions of TLR2, TLR4 and MyD88 in CTI are positively correlated with the degree of tissue inflammation. (2) The expression of TLR4 is more significant than that of TLR2 and MyD88 in onset of CTI. CTI is the initiating factor.