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在一个已构建的以细胞肌醇磷脂信号转导途径为原型的仿生信号转导系统中,处于合成肽脂质囊泡表面的乳酸脱氢酶LDH被激活后,催化乳酸脱氢反应过程中,使辅酶NADH氧化为NAD,在此过程中引入DPIP.利用DPIP,在囊泡表面形成了一个由机械信号到光信号转换的化学振荡体系,该体系以DPIP和NADH摩尔比为1∶10的比例进行振荡,其平均循环周期为32min,大于无肽脂质囊泡存在的体系之平均循环周期时间。结果表明:囊泡表面引入DPIP后,在整个系统受到机械刺激信号时,可以引发一个溶液从蓝色到无色,再由无色到蓝色,循环往复的化学振荡.由于LDH结合在囊泡表面,这可能降低了其对NADH的活化作用,从而令化学振荡的平均循环周期时间加长.
In a constructed biomimetic signal transduction system based on the phosphoinositide signal transduction pathway, lactate dehydrogenase (LDH) on the surface of synthetic lipid vesicles is activated to catalyze the dehydrogenation of lactate. The oxidation of coenzyme NADH to NAD, in the process of introducing DPIP.Using DPIP, a chemical oscillatory system was formed on the surface of the vesicles by the mechanical signal to optical signal conversion, the system DPIP and NADH molar ratio of 1:10 ratio Oscillating, with an average cycle time of 32 min, is greater than the average cycle time of the system in which the peptide-free lipid vesicles are present. The results showed that after DPIP was introduced into the surface of the vesicle, the whole system was stimulated by mechanical stimulus, which could initiate a solution from blue to colorless, and then from colorless to blue, and the cycle of chemical oscillation. As LDH binding in the vesicles Surface, which may reduce its activation of NADH, thereby lengthening the average cycle time of chemical oscillations.