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目的构建乙型肝炎病毒免疫吸附柱。方法以琼脂糖凝胶为载体,经活化后键合人抗-HBs-IgG。构建流程为:环氧介质的合成、氨基介质的合成、醛基介质的合成、免疫吸附介质的合成、未反应醛基的封尾、装柱。体外观察其对含HBV和HBsAg血浆的吸附作用。结果活化琼脂糖凝胶与抗-HBs-IgG的键合率为85.07%,患者血浆体外吸附实验结果表明,该免疫吸附柱可吸附、除去离体血浆中58.97%的HBsAg和53.1%的HBV颗粒。结论乙型肝炎病毒免疫吸附柱可吸附除去离体血浆中部分HBsAg和HBV颗粒。
Objective To construct a hepatitis B virus immunosorbent column. Methods Agarose gel was used as a carrier to bind human anti-HBs-IgG after activation. The construction process is as follows: synthesis of epoxy medium, synthesis of amino medium, synthesis of aldehyde medium, synthesis of immunoadsorption medium, sealing of unreacted aldehyde groups and packing. In vitro observation of its adsorption of HBV and HBsAg plasma. Results The binding rate of activated sepharose to anti-HBs-IgG was 85.07%. The results of in-vitro plasma adsorption showed that the immunosorbent column could adsorb and remove 58.97% of HBsAg and 53.1% of HBV particles in isolated plasma . Conclusion Hepatitis B virus immunoadsorption column can adsorb and remove some of the isolated plasma HBsAg and HBV particles.