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目的建立不同产地皂角刺HPLC指纹图谱,为皂角刺质量评价提供科学依据。方法采用RP-HPLC法,色谱柱为Agilent ZORBAX SB-Aq C_(18)(250 mm×4.6 mm,5μm),流动相为甲醇(A)-体积分数为0.1%的乙酸溶液(B),梯度洗脱,检测波长为254 nm,柱温为35℃,流速为1.0 m L·min-1。采用中药色谱指纹图谱相似度评价系统(2012.130723版本)和SPSS 21.0软件对谱图分别进行相似度分析和聚类分析。结果建立了皂角刺HPLC指纹图谱,确定了29个共有峰,并指认了其中5个共有峰的化学成分。从HPLC指纹图谱结果看,不同产地皂角刺化学成分相似,但含量差异较大。结论中药皂角刺HPLC指纹图谱可为中药皂角刺质量评价提供科学依据。
Objective To establish HPLC fingerprinting of Gleditsia sinensis in different habitats to provide a scientific basis for glitter quality evaluation. Methods The RP-HPLC method was used. The mobile phase consisted of methanol (A) -acetic acid solution (B) with a volume fraction of 0.1% and the mobile phase consisted of Agilent ZORBAX SB-Aq C 18 The detection wavelength was 254 nm, the column temperature was 35 ℃ and the flow rate was 1.0 m L · min-1. Similarity analysis and clustering analysis were carried out on the chromatogram by using the similarity evaluation system of Chinese medicine chromatographic fingerprint (2012.130723 version) and SPSS 21.0 software. Results The soap fingerprinting HPLC fingerprinting was established, the 29 common peaks were identified and the chemical components of five common peaks were identified. From the HPLC fingerprinting results, the chemical constituents of the different regions of the saponin spikes are similar, but the content is quite different. Conclusion The HPLC fingerprinting of Ginkgo biloba leaves by traditional Chinese medicine can provide a scientific basis for the quality evaluation of Gleditsia sinensis.