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以异硫氰四甲基若丹明－鬼笔环肽（ＴＲＩＴＣ－ＰＨＡＬＬＯＩＤＩＮ）为探针，对小麦根尖三个不同发育区－－分生区、伸长区和成熟区细胞中肌动蛋白纤丝（ＡＦｓ，亦称微丝ｍｉｃｒｏｆｉｌａｍｅｎｔ）的分布格局进行荧光显微观察和分析．结果表明：分生区细胞中细胞核为ＡＦｓ网络包围，细胞周缘有ＡＦＳ密集分布，胞质皮层中有ＡＦｓ广泛均匀的分布；伸长区细胞中细胞核亦为网络包围，并明显可见ＡＦｓ成束由核周围向四处辐射直到细胞周质，且与分布于细胞边缘的ＡＦｓ汇集；成熟区细胞中伴随着核移向细胞的一侧，中央大液泡的形成，胞质皮层中只可见较微弱的荧光，但亦可见在核周围和细胞膜附近分布稍多．总之，随着小麦很尖细胞从分生到分化，细胞内ＡＦｓ的分布格局发生了变化，且这种变化与细胞所处的状态及生理功能相关．
Using TRITC-PHALLOIDIN as probe, we detected the expression of actin in three different developmental zones of apical meristem, meristematic zone, elongation zone and mature zone Filaments (AFs, also known as microfilament) distribution pattern fluorescence microscopy and analysis. The results showed that the nuclei in the meristematic zone were surrounded by the AFs network, the AFS was densely distributed on the periphery of the cells, and the distribution of AFs in the cytoplasmic cortex was extensive and uniform. The nuclei in the elongation zone were also surrounded by the network, Nuclear radiation around four weeks until the cell periplasm, and distributed in the edge of the cell aggregation AFs; mature cells along with the nucleus to the cell side, the formation of the central vacuole, only visible in the cytoplasm cortex weaker fluorescence , But also seen around the nucleus and membrane distribution slightly more. In conclusion, the distribution pattern of intracellular AFs has changed as the number of spiked wheat cells varies from vegetative to physiological, and this change is related to the state and physiological function of the cells.