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连接有蒸发光散射检测器的高速逆流色谱仪首次成功的应用于制备和分离青葙子中的皂苷celosins A和B。二氯甲烷:正丁醇∶甲醇∶水(4∶0.3∶3∶2)+0.5%冰醋酸作为洗脱溶剂系统。从半制备型HSCCC收集到的组分进行HPLC分析,可以得到:celosin A纯度为98.9%,celosin B的纯度为98.1%。这是高速逆流色谱仪首次被用于纯化青葙子中的皂苷,两个化合物的结构通过碳谱和质谱来确定。
High-speed countercurrent chromatography coupled with an evaporative light scattering detector was successfully used for the first time in the preparation and isolation of the saponins celosins A and B in the barley. Dichloromethane: n-butanol: methanol: water (4: 0.3: 3: 2) + 0.5% glacial acetic acid as elution solvent system. HPLC analysis of the fractions collected from the semi-preparative HSCCC yielded: celosin A with a purity of 98.9% and celosin B with a purity of 98.1%. This is the first time that a high-speed countercurrent chromatograph has been used to purify the saponin from the barley. The structure of the two compounds is determined by carbon and mass spectrometry.