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以东方百合“西伯利亚”为试验材料,研究百合无症病毒(LSV)侵染百合对其叶片生理生化以及叶绿体超微结构的影响。检测结果表明:叶片中叶绿素a、b以及总叶绿素含量与健康对照相比分别下降了28.6%、33.3%和23.5%,净光合速率、气孔导度及胞间CO_2浓度分别下降33.3%、25%和13.8%;超氧化物歧化酶(SOD)、过氧化物酶(POD)、多酚氧化酶(PPO)和苯丙氨酸解氨酶(PAL)与健康对照相比,分别增加了16.6%、29.4%、16.7%和22.2%。电镜观察发现:感病植株叶绿体膨胀变形,基质片层散乱,叶绿体内淀粉粒肿大且数目增多,从而证明LSV侵染破坏叶绿体结构,影响植株的光合作用。
The effects of lily infestation lily (Lily) on its leaf physiological and biochemical characteristics and the chloroplast ultrastructure were studied using Oriental Lily “Siberian ” as experimental materials. The results showed that the content of chlorophyll a, b and total chlorophyll in leaves decreased by 28.6%, 33.3% and 23.5%, the net photosynthetic rate, stomatal conductance and intercellular CO_2 decreased by 33.3%, 25% And 13.8% respectively. Compared with healthy controls, the contents of superoxide dismutase (SOD), peroxidase (POD), polyphenol oxidase (PPO) and phenylalanine ammonia lyase (PAL) increased by 16.6% , 29.4%, 16.7% and 22.2%. Electron microscopy showed that the chloroplasts of diseased plants expanded and deformed, the matrix lamella was scattered, and the number of starch granules in the chloroplast was large and increased. Thus, LSV infection could destroy the chloroplast structure and affect the photosynthesis of the plants.