Streptomyces rubrogriseus HDZ-9-47,isolated from eggs of Meloidogyne spp.,was evaluated as a potential biocontrol agent of Meloidogyne incognita under in vitro and protective field.Microscopic observations showed that HDZ-9-47 parasitized eggs of M.incognita within 7 days.In vitro,the culture filtrate of HDZ-9-47 caused 97.0%mortality of second-stage juveniles(J2s)of M.incognita and inhibited more than 50%egg hatching.In the field,compared with the control,the rootknot index and J2s density in the treatment of drench the broth contained 1012 HDZ-9-47 spores were respectively reduced by 51.1 and 80.7%at 90 days post transplantation,which were better than that in other application doses and methods.In addition,reduction rates of root-knot index and J2s density of the treatment of combined application of HDZ-9-47 with biofumigation was 87.1 and 91.0%,respectively,better than either of HDZ-9-47 or biofumigation used alone or fosthiazate treatment.And tomato yield also increased by 16.1%.Together,our results suggest that HDZ-9-47 could be an effective biocontrol agent of M.incognita,and that application of HDZ-9-47 combined with cabbage residue biofumigation was a promising and sustainable option for M.incognita control. Streptomyces rubrogriseus HDZ-9-47, isolated from eggs of Meloidogyne spp., Was evaluated as a potential biocontrol agent of Meloidogyne incognita under in vitro and protective field. Microscopic SCIEN示 showed that HDZ-9-47 parasitized eggs of M. incognita within 7 the culture filtrate of HDZ-9-47 caused 97.0% mortality of second-stage juveniles (J2s) of M.incognita and inhibited more than 50% egg hatching. In the field, compared with the control, the rootknot index and J2s density in the treatment of drench the broth contained 1012 HDZ-9-47 spores were respectively reduced by 51.1 and 80.7% at 90 days post transplantation, which were better than that in other application doses and methods. of root-knot index and J2s density of the treatment of combined application of HDZ-9-47 with biofuction was 87.1 and 91.0%, respectively, better than either of HDZ-9-47 or biofumigation used alone or fosthiazate treatment. And tomato yield also increased by 16.1% .To gether, our results suggest that HDZ-9-47 could be an effective biocontrol agent of M.incognita, and that application of HDZ-9-47 combined with cabbage residue biofumigation was a promising and sustainable option for M.incognita control.