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近年来,已有不少有关抗独特型抗体作为受体探针、免疫原及疫苗等方面探讨的报道。本文作者制备了抗柯萨奇病毒B_4(CB_4)的三种不同单克隆抗体(Ab_1)的抗独特型抗体(Ab_2)。Ab_2有抑制Ab_1中和CB_4的作用,并能诱生抗-抗独特型抗体(Ab_3)。为检测它们与细胞受体结合的能力,将BGMK或HeLa-S_3细胞与Ab_2在37℃孵育1小时后,加入~3H亮氨酸及~3H尿嘧啶放射标记的CB_4。病毒与细胞结合的百分率在实验组与正常血清对照组无显著差异。即抗独特型抗体不抑制CB_4与细胞结合的能力。
In recent years, many anti-idiotypic antibodies have been reported as receptor probes, immunogens and vaccines. The authors prepared anti-idiotypic antibodies (Ab_2) against three different monoclonal antibodies (Ab_1) against Coxsackie virus B_4 (CB_4). Ab_2 inhibited the binding of Ab_1 to CB_4 and induced anti-idiotypic antibody (Ab_3). To test their ability to bind to cellular receptors, BGMK or HeLa-S_3 cells were incubated with Ab_2 for 1 hour at 37 ° C and ~ 3H leucine and ~ 3H uracil radiolabeled CB_4 were added. The percentage of virus-cell binding was not significantly different between the experimental group and the normal serum control group. That is, anti-idiotypic antibodies do not inhibit the ability of CB4 to bind to cells.