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目的研究粒细胞-巨噬细胞集落刺激因子(GM-CSF)转基因小鼠经中子照后肺脏和肝脏中血管内皮细胞生长因子(VEGF)蛋白的表达变化。方法 BALB/C小鼠进行全身中子照射,吸收剂量为0.6Gy,分为未转基因组和转基因组,转基因组于照射前24 h进行hGM-CSF活体基因电转染。两组小鼠照射后1、14和28 d分批活杀,应用HE染色观察组织病理形态学变化,免疫组化法检测VEGF的蛋白表达及用Western blot研究VEGF在肺脏和肝脏中的表达变化。结果转基因组小鼠在照后14～28 d,肺脏及肝脏病理损伤较轻,VEGF蛋白表达水平较未转基因组增高。结论 GM-CSF基因活体转染对小鼠中子急性损伤极期和恢复期血管生成和恢复具有促进作用。
Objective To investigate the expression of vascular endothelial growth factor (VEGF) in the lung and liver after neutron irradiation of granulocyte-macrophage colony-stimulating factor (GM-CSF) transgenic mice. Methods BALB / C mice were immunized with whole body neutrons at a dose of 0.6Gy, which were divided into non-transgenic group and transgenic group. Transgenic group was transfected with live hGM-CSF gene 24 hours before irradiation. The mice were killed in batches on day 1, 14 and 28 after irradiation. The histopathological changes were observed by HE staining. The protein expression of VEGF was detected by immunohistochemistry and the expression of VEGF in lung and liver . Results In the 14th to 28th day after irradiation, the mice in the transgenic group showed less pathological damage in the lung and liver and the expression of VEGF protein was higher than that in the non-transgenic group. Conclusion Transfection of GM-CSF gene in vivo can promote the neovascularization and recovery of neutron acute injury in both polar and convalescent phases.