论文部分内容阅读
采用根癌农杆菌AGL-1介导的方法,对生防青霉Penicillium striatisporum Pst10进行绿/黄色荧光蛋白-遗传霉素抗性双标记,研究标记前后菌株的生长差异以及拮抗性能的变化。以Pst10的新鲜菌丝体为受体,获得12个青霉Pst10的绿色荧光蛋白-遗传霉素抗性(GFP-Gen)双标记菌株,转化率0.24%;获得14个青霉Pst10的黄色荧光蛋白-遗传霉素(YFP-Gen)抗性双标记菌株,转化率0.28%。在所获得的转化子中,部分双标记转化子的生长速度和拮抗性能有不同程度的改变,分别筛选获得1株和3株生长速度和拮抗性能与野生型没有明显差异的GFP-Gen和YFP-Gen双标记转化子。
The Agrobacterium tumefaciens AGL-1-mediated method was used to double-stain green / yellow fluorescent protein-geneticin resistant Penicillium striatisporum Pst10 to study the differences in growth and antagonistic properties of the strains. Twelve Penicillium Pst10 green fluorescent protein-geneticin resistant (GFP-Gen) double-labeled strains were obtained with fresh mycelia of Pst10 as recipients at a conversion rate of 0.24%. Fourteen P. fluorescens Pst10 yellow fluorescence Protein-geneticin (YFP-Gen) resistant double marker strain with a conversion rate of 0.28%. Among the obtained transformants, the growth rate and antagonistic performance of some double-labeled transformants varied to some extent. One strain and three strains of GFP-Gen and YFP which had no significant difference in growth rate and antagonism with wild type -Gen double-labeled transformant.