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目的:研究红参水提液对运动小鼠的抗疲劳作用及其机制。方法:制备每1 ml水提液相当于0.022 g红参药材的水提液。将小鼠按体重分为空白对照组及红参水提物低、中、高剂量组，每组12只。红参水提物低、中、高剂量组灌胃红参水提物15、30、45 ml/kg，空白对照组灌胃等体积蒸馏水，1次/d，连续灌胃30 d。测定小鼠负重游泳时间、肌肉组织ATP酶活力、血清SOD和MDA水平、血清BUN含量及LDH活力、肝脏肝糖原含量及运动后血乳酸含量等指标。结果:与空白对照组比较，红参水提液高剂量组负重游泳时间[(661.35±245.62)s比(412.23±141.35)s]延长(n P<0.05)；肌肉组织Nan ＋-Kn ＋-ATP酶[(4.73±1.21)U/mg比(3.43±1.07)U/mg]和Can 2＋-Mgn 2＋-ATP酶活力[(4.87±1.54)U/mg比(3.98±1.12)U/mg]升高(n P<0.05)；血清SOD水平[(102.54±18.73)U/ml比(70.37±8.69)U/ml]升高(n P<0.05)，MDA水平[(8.36±0.55)nmol/ml比(15.73±1.18)nmol/ml]降低(n P<0.05)；血清LDH活力[(5 718.72±716.35)U/L比(4 824.91±532.67)U/L]升高(n P<0.01)，肝脏肝糖原量[(6 149.74±1 236.65)mg/100 g比(4 696.27±555.63)mg/100 g]升高(n P<0.05)；红参水提液中、高剂量组血乳酸含量[(1.86±0.52)mmol/L、(1.78±0.46)mmol/L比(2.89±1.44)mmol/L]降低(n P<0.05)，且血乳酸曲线下面积[(89.79±17.81)及(84.83±14.87)比(105.25±22.44)]降低(n P<0.05)。n 结论:红参水提液可延长疲劳小鼠负重游泳时间，增加肝糖原积累，降低血清乳酸含量，并通过抗氧化作用增强机体对运动负荷的适应能力，发挥抗疲劳作用。“,”Objective:To investigate the antifatigue effect and mechanism of n Ginseng Radix et Rhizoma Rubra aqueous extract on mice after exercise.n Methods:The aqueous extract of n Ginseng Radix et Rhizoma Rubra was acquired by water extraction and concentration, and the aqueous extract 1 ml was equivalent to raw material 0.022 g. The mice were randomly divided into blank control group, low dose group, middle dose group and high dose group of n Ginseng Radix et Rhizoma Rubra aqueous extract, 12 mice in each group. In the low, medium and high dose groups of n Ginseng Radix et Rhizoma Rubra aqueous extract, 15, 30 and 45 ml/kg of n Ginseng Radix et Rhizoma Rubra aqueous extract were given by gavage, and the blank control group was given an equal volume of distilled water once a day. After intragastric infusion for 30 consecutive days, the antifatigue effect and mechanism of n Ginseng Radix et Rhizoma Rubra was evaluated by load swimming time, ATPase activity in muscle tissue, serum superoxide dismutase (SOD) and malondialdehyde (MDA) level, the content of serum urea nitrogen and hepatic glycongen content, activity of serum lactate dehydrogenase and blood lactic acid content of the mice after exercise.n Results:Compared with the blank control group, the load swimming time (661.35 ± 245.62 s n vs. 412.23 ± 141.35 s) of the high dose group was significantly prolonged (n P<0.05); Nan + -Kn + -ATPase (4.73 ± 1.21 U/mg n vs. 3.43 ± 1.07 U/mg) and Can 2+ -Mgn 2+ - ATPase activity (4.87 ± 1.54 U/mg n vs. 3.98 ± 1.12 U/mg) in muscle tissues of the high dose group were both significantly increased (n P<0.05); serum SOD level (102.54 ± 18.73 U/mln vs. 70.37 ± 8.69 U/ml) in high dose group was increased (n P<0.05), and MDA level (8.36 ± 0.55 nmol/mln vs. 15.73 ± 1.18 nmol/ml) was decreased (n P<0.05); serum lactate dehydrogenase activity (5 718.72 ± 716.35 U/Ln vs. 4 824.91 ± 532.67 U/L) of the high dose group was increased (n P<0.05), and hepatic glycogen content [(6 149.74 ± 1 236.65) mg/100 gn vs. (4 696.27 ± 555.63) mg/100 g] of the high dose group was increased (n P<0.05); the content of blood lactic acid (1.86 ± 0.52 mmol/L, 1.78 ± 0.46 mmol/Ln vs. 2.89 ± 1.44 mmol/L) in the medium and high dose group were both significantly reduced (n P<0.05) , and the area under the blood lactate curve (89.79 ± 17.81, 84.83 ± 14.87n vs. 105.25 ± 22.44) were also significantly reduced (n P<0.05).n Conclusions:Ginseng Radix et Rhizoma Rubra aqueous extract could prolong the load swimming time, increase liver glycogen accumulation, reduce blood lactic acid content, and enhance the ability to adapt the exercise load through anti-oxidation function.n